摘要
目的:建立五味渣驯丸中羟基红花黄色素A、没食子酸、马兜铃酸A的含量测定方法。方法:采用HPLC对制剂中主要成分羟基红花黄色素A、没食子酸、马兜铃酸A进行定量分析。CAPCELL PAK C18色谱柱(4.6 mm×250 mm,5μm),甲醇-0.4%磷酸(30∶70)、甲醇-0.2%磷酸(7∶93)、甲醇-0.05%冰醋酸(79∶21)为流动相,流速1.0 mL.min-1,检测波长为403,273,390 nm。结果:羟基红花黄色素A、没食子酸、马兜铃酸A的线性范围分别为0.116～1.740μg(r=0.999 97),0.067～0.673μg(r=0.999 95),0.024～0.247μg(r=0.999 99);平均回收率分别为98.62%(RSD 2.15%),100.01%(RSD 2.03%),96.83%(RSD 1.74%)。结论:本方法简便、结果准确可靠,可有效控制制剂质量。
Objective：To establish an method for determination of hydroxysafflor yellow A,gallic acid and aristolochic acid A in Wuwei Zhaxun pill.Method：The hydroxysafflor yellow A,gallic acid and aristolochic acid A in Wuwei Zhaxun pill was determined by HPLC.CAPCELL PAK C18（4.6 mm×250 mm,5 μm）column was used and detection wavelength was at 403,273,390 nm.The mobile phase was methanol-0.4% phosphoric acid（30∶70）,methanol-0.2%phosphoric acid（7∶93）,methanol-0.05% glacial acetic acid（79∶21）,with the flow rate of 1.0 mL · min-1.Result：The calibration curves of hydroxysafflor yellow A,gallic acid and aristolochic acid A were in a good linearity over the ranges of 0.116-1.740 μg（r=0.999 97）,0.067-0.673 μg（r=0.999 95）,0.024-0.247 μg（r=0.999 99）,respectively.The average recoveries were 98.62%（RSD 2.15%）,100.01%（RSD 2.03%）,96.83%（RSD 1.74%）.Conclusion：The method is simple,accurate and can be used for the control.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第18期109-112,共4页
Chinese Journal of Experimental Traditional Medical Formulae
作者简介
倪琳，本科，主管药师，从事中成药、藏药质量分析及检验工作，Tel：09314968949．E-mail：nilinmanager937@sohu．com
