摘要
目的 HPLC法测定单叶细辛中马兜铃酸A的含量。方法采用Sunfire C18柱（150mm×4.6mm,5μm）;流动相：甲醇-水-醋酸（60∶36∶4）;检测波长316nm。结果线性范围为6.60~39.60μg·mL^-1（r=0.9995）,平均回收率为95.8%,RSD为0.8%（n=6）。结论该方法简便、准确,可用于单叶细辛中马兜铃酸A的质量控制。
OBJECTIVE To establish a method for determination of aristolochic acid A in Asarum himalaicum.METHODS The conditions of HPLC were Sunfire C18 column（150mm×4.6mm,5μm）,methanol-water-acetic（60∶36∶4） as mobile phase.The detection wavelength was 316nm.RESULTS The linear range was 6.60～39.60μg·mL^-1（r=0.9995）,the average recovery was 95.8%,and the RSD was 0.8%（n=6）.CONCLUSION The medthod was simple,accurate and can be used to control quality of aristolochic acid A in Asarum himalaicum.
出处
《齐鲁药事》
2010年第10期593-595,共3页
qilu pharmaceutical affairs
