摘要
目的通过比较导赤散及其配伍组中马兜铃酸A的含量,探索方剂配伍减毒的作用。方法采用色谱法,色谱柱为D iamon TmC18(250mm×4.6mm,5μm),甲醇-水-冰乙酸(72∶27∶1)为流动相;柱温:室温;检测波长315nm;流速1.0mL/m in。结果关木通组、导赤散组、配伍一组、配伍二组的马兜铃酸A含量分别为:0.549mg/g、0.357mg/g、0.226mg/g、0.316mg/g。结论生药关木通经过配伍后,马兜铃酸A的含量能显著降低。
Objective To explore the toxitcity - reducing effect in prescription compatibility via comparison on content of aristolochic acid A between Daochi powder and its components group. Methods Chromatography was applied in this study, with Diamon TmC18 （250mm ×4.6mm, 5μm ） used as chromatographic column, methanol -water-glacial acetic acid （72：27：1） as mobile phase and room temperature selected as the column temperature, a parameter 315 nm as the detection wavelength and a 1.0mL/min as the velocity. Results Contents of aristolechic acid A were detected as： 0.549mg/g in Guanmntong （caulis hocquartiac） group, 0.357mg/g in Daochi powder group, 0.226mg/g in combination group one and 0.316mg/g in combination group 2. Conclusion Content of aristoleehic acid A declined notably after the crude caulis hocquartiae was combinafively used.
出处
《湖北中医学院学报》
2009年第3期30-31,共2页
Journal of Hubei College of Traditional Chinese Medicine
基金
湖北中医学院资助项目(中医科(2005)11号)
关键词
关木通
马兜铃酸A
高效液相色谱法
导赤散
配伍
Caulis Aristoloehiae Maushuriensis
Aristolochic Acid A
High Performance Liquid Chromatography
Daochi powder
Combination/ Compatibility
作者简介
吴建红（1960-），女，湖北中医学院基础医学院副教授。
