摘要
目的:探讨人胰岛素及其类似物对人乳腺癌细胞系(MCF-7)增殖的影响及二甲双胍联合胰岛素及其类似物对MCF-7细胞增殖的影响。方法:①使用不同浓度(0、1、10、100、1 000 nmol/L)的人胰岛素及类似物干预MCF-7细胞72 h后,用CCK-8法检测干预后细胞增殖情况。②用胰岛素及类似物(每种胰岛素浓度均为1 000 nmol/L)干预MCF-7细胞,分别用CCK-8法检测各胰岛素在干预24、48、72 h后的细胞增殖。③用CCK-8法检测单纯二甲双胍(0、2.5、5.0、10.0、20.0 mmol/L),及二甲双胍联合胰岛素及其类似物(浓度均为1 000 nmol/L)干预MCF-7 72 h后细胞的增殖情况。结果:人胰岛素及类似物均能促进乳腺癌细胞系增殖并呈时间依赖性,人胰岛素、甘精胰岛素、赖脯胰岛素对细胞的增殖作用呈显著的剂量依赖性,相同条件下不同胰岛素对细胞的增殖作用无明显差异,但甘精胰岛素、赖脯胰岛素在相同条件下促细胞增殖作用高于其他胰岛素。二甲双胍可以呈浓度依赖性地抑制细胞的增殖作用,并减弱胰岛素类似物引起的细胞增殖作用,相同浓度二甲双胍对不同胰岛素类似物促增殖的抑制作用无明显差异,但对地特胰岛素的抑制作用小于其他胰岛素类似物。结论:胰岛素类似物和人胰岛素均能促进肿瘤细胞的增殖,二甲双胍可以抑制MCF-7细胞增殖,二甲双胍可以拮抗胰岛素及其类似物引起的促细胞增殖作用。
Objective：To investigate the effect of human insulin and insulin analogues combined with or without mefformin on the proliferation of human mammary carcinoma cell line MCF-7. Methods： Firstly,human mammary carcinoma cell line （MCF-7） was treated with different concentrations （1,10,100, 1 000 nmol/L） of insulin analogues or human insulin. Secondly,MCF-7 cells was treated with insulin analogues or human insulin at the concentration of 1 000 nmol/L,and the cell proliferation was tested by CCK-8 assay after 24 h,48 h and 72 h treatment. Thirdly, MCF-7 cells were treated with different concentrations （0,2.5,5,10,20 mmol/L） of metformin combined with human insulin or insulin analogues （1 000 nmol/L each）. The proliferation of MCF-7 cells was also examined by CCK-8 assay. Results： Both insulin analogues and human insulin could increase the proliferation of cell line MCF-7. Glargine, Lispro and human insulin had a dose-and time-dependent effect on the cell proliferation while Detemir and Aspart had only a time-dependent effect. Metfomin could inhibit the proliferation of MCF-7 cells in a dose-dependent response. Metformin could decrease the effect on the proliferation that was induced by human insulin and insulin analogues. Conclusion： Human insulin and insulin analogues couid increase the proliferation of human mammary carcinoma cell line MCF-7. Metformin could decrease the proliferation of MCF-7 induced by human insulin and insulin analogues.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第7期923-927,共5页
Journal of Nanjing Medical University(Natural Sciences)
