摘要
目的探讨不同人群外周血中乳腺小黏蛋白(small breast epithelial mucin,SBEM)水平以及乳腺癌患者外周血中SBEM水平与病理因素的关系和临床意义。方法收集经病理证实的68例乳腺癌及20例乳腺纤维腺瘤和20例健康志愿者。采用酶联免疫吸附测定法(ELISA)检测3组不同人群血清SBEM水平。分析乳腺癌患者外周血中SBEM水平与病理因素的关系。3组均抽取空腹静脉血3ml。采集血样前乳腺癌患者及纤维腺瘤组患者均未接受放疗、化疗或生物治疗等干预措施及有创检查。统计分析,符合正态分布的,两组间比较采用独立样本t检验,多组间比较行方差分析,LSD法进行两两比较。不符合正态分布的多组比较采用Kruskal-Wallis H检验。结果 3组间血清SBEM水平差异存在显著的统计学意义(P<0.050)。乳腺癌组血清SBEM水平均高于纤维腺瘤组和健康志愿者组(P均<0.001)。纤维腺瘤组与健康志愿者组相比,差异无统计学意义(P>0.050)。乳腺癌患者的血清SBEM水平与淋巴结转移状况和TNM分期有关(P均<0.001),与患者的年龄、月经状况、病理类型、组织学分级、肿瘤大小、ER、PR、HER-2、P53、Ki-67、基因分型无关(P均>0.050)。乳腺癌SBEM血清水平随淋巴结分期增加而升高,不同淋巴结分期间的SBEM水平差异有显著的统计学意义(P<0.001)。结论 SBEM特异性表达于乳腺癌外周血。乳腺癌患者外周血SBEM水平在一定程度上能够反映临床分期和腋窝淋巴结情况,有望成为检测乳腺癌外周血微转移的标志物和判断乳腺癌病情发展和预后的指标。
Objective To explore the level of small breast epithelial mucin （SBEM） in peripheral blood in different populations, and its association with pathological factors of breast cancer patients as well as the clinical significance. Methods Pathologically confirmed 68 breast cancer and 20 mammary fibroadenoma patients and 20 healthy volunteers were enrolled in the study. Enzyme-linked immunosorbent assay was used to detect the serum SBEM level in the three different populations. The association of the serum SBEM level with pathological factors of breast cancer patients was analyzed. Empty stomach vein blood of 3 ml was obtained. Before blood sample collection, breast cancer and fibroadenoma patients did not have any administration of radiotherapy, chemotherapy, bioremediation or any invasive examinations. Statistical analysis was done using t test for comparison of two groups, one-way variance analysis for more groups and LSD for multiple comparison, and Kruskal-Wallis test for comparison of groups of non-normal distribution and Mann-Whitney test for multiple comparison. Results There was statistical difference in the serum SBEM level between the three groups （P〈 0. 050 ）. The serum SBEM level in the breast cancer group was higher than in both the fibroadenoma group and the volunteer group（ P〈0. 001 ）, but there was no statistical difference between the fibroadenoma group and the volunteer group（P〉0. 050）. In breast cancer patients, the serum SBEM level was correlated with axillary lymph nodes status and TNM stage （（P〈0. 001）, but not correlated with the age, menstruation condition, pathological type, histological grade, tumor maximum diameter, estrogen receptor, progesterone receptor, human epidermal growth factor receptor 2, p53 protein, Ki-67, and gene subtypes （all P〉0. 050）. The serum SBEM level increased with increase of stage of lymph nodes, and between different lymph node stages there was statistical difference in the serum SBEM （P〈0. 001 ） in breast cancer patients. Conclusion SBEM specifically expresses in the peripheral blood of breast cancer patients. The serum SBEM level in breast cancer patients can reflect clinical stage and lymph node status to a certain extent. SBEM is a promising marker detecting micrometastasis and judging progression and prognosis of breast cancer.
出处
《中华乳腺病杂志（电子版）》
CAS
2012年第2期12-16,共5页
Chinese Journal of Breast Disease(Electronic Edition)
基金
2011年兰州市科技局社会发展基金资助项目(20110236)
关键词
乳腺肿瘤
乳腺小粘蛋白
酶联免疫吸附试验
外周血
疾病进展
breast neoplasms
small breast epithelial mucin
enzyme-linked immunosorbent assay
peripheral blood
disease progression
