摘要
目的探讨乳腺癌患者骨髓中人乳腺小黏蛋白(HSBEM)mRNA的表达及其临床意义。方法用RT-PCR技术检测68例乳腺癌患者、17例乳腺良性肿瘤患者以及11位健康人骨髓中的HSBEM mRNA表达,并分析HSBEM mRNA表达与临床病理因素之间的关系。结果68例乳腺癌患者中,24例检测出HSBEM mRNA阳性表达,阳性表达率为35.3%(24/68)。乳腺良性肿瘤患者和健康人骨髓中未检测到HSBEM mRNA表达。HSBEM mRNA阳性表达与乳腺癌临床分期和腋窝淋巴结转移状况有关(P<0.05);与年龄、肿瘤大小、ER和PR表达状况差异无统计学意义(P>0.05)。结论HSBEM mRNA可作为检测乳腺癌患者骨髓微转移的分子指标之一。骨髓微转移的检测可为乳腺癌患者的治疗和预后判断提供帮助。
[ Objective ] To evaluate expression and clinical significance of the human small breast epithelial mucin （HSBEM） mRNA in bone marrow of breast cancer patients. [Methods] Expression of HSBEM mRNA was detected by the reverse transcriptase polymerase chain reaction （RT-PCR） in the bone marrow aspiration from 68 breast cancer patients,with samples of 17 breast benign tumor and 11 healthy volunteers as control. The possible correlation of HSBEM mRNA expression with clinicopathologie parameters and related molecular markers were analyzed. [Results] The positive expression of HSBEM mRNA was detected by RT-PCR in 24 of 68 patients with breast cancer. The positive rate was 42.7%. Negative expression of HSBEM mRNA was found in the bone marrow aspiration of patients with benign breast lesion and healthy volunteers. The HSBEM mRNA expression was positively correlated with clinical stage and axillary nodal involvement （P 〈0.05）. but was not related to age, tumor size, ER and PR status （P 〉0.05）. [ Conclusion ] The HSBEM mRNA may be used as a molecular marker to detect bone marrow micrometastasis （BMM） in patients with breast cancer. The resuits of detecting BMM may have an impact upon therapeutic recommendations and prognostic judgement in breast cancer patients.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2009年第22期3473-3476,共4页
China Journal of Modern Medicine
关键词
乳腺肿瘤
骨髓
人乳腺小黏蛋白
微转移
breast neoplasms
bone marrow
human small breast epithelial muein
mierometastases
