摘要
目的:建立合胃口服液中橙皮苷含量测定方法。方法:采用高效液相色谱法(HPLC)测定本品中橙皮苷的含量。ODS柱(250mm×4.6mm×5μm),甲醇-2%乙酸水溶液(39:61)为流动相,流速为1ml/min,检测波长为283nm。结果:橙皮苷在0.1344～1.6800μg含量呈线性关系,r=0.9998;加样回收率为98.44%,RSD=1.21%(n=6)。结论:本法灵敏、准确、重现性好,可作为合胃口服液的质量控制指标之一。
Objective： To establish the determination method for determining the content of hesperidin in combined stomach oral liquid. Methods： The high-performance liquid chromatography （HPLC） method was used to determine the content of hesperidin in the studied product. The Octadecylsilyl （ODS） column was 250 mm×4.6 mm×5 μm; the methanol-2% acetic acid solution （39∶61） presented mobile phase; the flow rate was 1 ml/min; the detection wavelength was 283 nm. Results： Hesperidin showed a linear relationship in the content of 0.134 4 to 1.680 0 μg, r=0.999 8. The sample recovery rate was 98.44%, RSD=1.21% （n=6）. Conclusion： This method is sensitive, accurate and reproducible, and therefore can be used as one of the quality control indicators of combined stomach oral liquid.
出处
《中国当代医药》
2012年第1期40-41,共2页
China Modern Medicine
作者简介
张克（1974-），男，职称：主管药师；工作单位：河南省中医药研究院附属医院。
