摘要
采用蛋白酶和纤维素酶对三孢布拉霉菌丝体细胞壁进行酶解处理,研究酶添加量、pH、温度和酶解时间等因素对破壁效果的影响。确定最佳工艺条件为:酶添加量为0.3%,pH为6.0,温度45℃,蛋白酶酶解4h后纤维素酶酶解2h,最终提取率达到90.2%。实现了低能、温和、环保的生产提取工艺,为番茄红素工业化清洁生产打下基础。
The protease and cellulase were used to break the cell wall of Blakeslea trispora,and the addition of enzyme,pH,temperature and enzymic hydrolysis time to cell disruption were studied.The optimal conditions were as follows：enzyme addition 0.3%,pH6.0,temperature 45℃ and protease hydrolysis time 4h,cellulase hydrolysis time 2h.The final extraction rate reached 90.2%.It realized low-energy,gentle,environmental production extraction technology,laid the foundation for lycopene industrialization cleaner production.
出处
《食品工业科技》
CAS
CSCD
北大核心
2012年第3期276-278,共3页
Science and Technology of Food Industry
关键词
番茄红素
提取
破壁
lycopene
extraction
cell broken
作者简介
万红贵（1955-），男，研究员，主要从事微生物发酵工程及放大研究。
