摘要
目的:研究番茄红素高压后的组分变化及通过体外研究,观察不同组分番茄红素的抗人乳腺癌细胞活性。方法:采用HPLC法和UV-vis法分析高压后番茄红素组分变化。采用噻唑蓝(MTT)法考察不同结构的番茄红素对雌激素受体阴性(ER-)人乳腺癌细胞MDA-MB-435S的增殖抑制作用。结果:(1)番茄红素高压处理(300MPa,8min,10℃)后总量变为原来的56.3%,顺式异构体比例从0.3%提高到10.05%。(2)番茄红素对MDA-MB-435S细胞的增殖抑制作用有剂量-效应关系和时间-效应关系。未处理番茄红素IC50为22.4μmol/L;高压后番茄红素IC50为13.6μmol/L。(3)高压后番茄红素对乳腺癌细胞的增殖抑制作用增强。在浓度为10μmol/L时,4d后,高压后番茄红素对细胞的抑制率是未处理番茄红素的1.9倍。结论:番茄红素顺式异构体的抗乳腺癌细胞活性大于反式异构体,有关机制值得进一步研究。
Objective： To study the isomeric ratio change of lycopene after high pressure processing （HPP） and its antiproliferation activity. Method： The change of lycopene isomers ratio after HPP was analyzed by HPLC and UV-vis. By means of MTF assay, the antiproliferation activities of lycopene with different isomer ratios on estrogen receptor negative （ER-） cell MDA-MB-435S were studied. Results： （1） Total lycopene content decreased to 56.3% after HPP （300 MPa, 8 min, 10℃ ）, however, the cis-isomer ratio increased from 0.3% to 10.05%. （2） The antiproliferation effect was both concentration and time dependent. IC50 of the untreated lycopene was 22.4μmo/L while the IC50 of the lycopene after HPP was 13.6μmo/L. （3） The antiproliferation effect of lycopene after HPP increased. The inhibition rate of lycopene after HPP was 1.9 times higher than that of the untreated .Conclusion： The antibreast cancer cell proliferation effect of cis isomer of lycopene was higher than that of all-trans isomer. Its mechanism deserves further study.
出处
《营养学报》
CAS
CSCD
北大核心
2005年第6期506-509,共4页
Acta Nutrimenta Sinica
基金
国家留学基金科研项目(No.留金法[1999]5003)
江苏省教育厅自然科学研究重点项目(No.02KJA550001)
关键词
超高压
番茄红素
顺反异构物
乳腺癌
增殖抑制
high pressure processing
lycopene
cis and trans isomers, mammary cancer
antiproliferation
作者简介
邱伟芬（1965-），女，博士在读，副教授；E-mail：wfqiu@jlonline．com
