摘要
目的:分析比较铁皮石斛及常见伪品中多糖和甘露糖的含量。方法:采用高效液相色谱法测定石斛中甘露糖的含量,紫外-可见分光光度法测定多糖含量。结果:分析表明,各个不同的石斛药材多糖和甘露糖含量存在较大的差异。多糖含量最高的是齿瓣石斛,含量为44.43%;其次是报春石斛,含量为41.08%;含量最低的为细茎石斛,含量20.23%。甘露糖含量中,钩状石斛居首位,含量为34.17%;其次是齿瓣石斛,含量为32.96%;含量最低的为细茎石斛,含量为17.60%。结论:不同来源的铁皮石斛多糖和甘露糖含量均能达到2010年版《中国药典》要求,因此,传统以多糖和甘露糖为指标评价石斛质量是否合理有待进一步深入研究。
Objective：To compare the content of polysaccharide and mannose in different kinds of dendrobium candium.Methods：Mannose in different types of dendrobium candium can be determined by HPLC and polysaccharide carried out by UV.Results：The analysis showed that the contents of polysaccharide and mannose in different kinds of dendrobium candium have obvious difference.The highest content of polysaccharide is the D.devonianum Paxt,which contains 44.43%;then the second one is the D.primulinum Lindl which contains 41.08%;the lowest is D.moniliforme,which only contains 22.30%.However,mannose content of D.aduncum Lindl.is highest,which contents 34.17%,the second is 32.96% in D.devonianum Paxt,the lowest for D.moniliformes is 17.60%.Conclusion：The content of polysaccharide and mannose in D.officinale Kimura et Migo from different sources can reach the 2010 edition of Chinese Pharmacopoeia requirement.Therefore,the conventional indicators of Polysaccharides and Mannose to evaluate the quality of Dendrobium is not reasonable.Further research should be developed.
出处
《中华中医药学刊》
CAS
2012年第1期146-148,共3页
Chinese Archives of Traditional Chinese Medicine
基金
国家科技支撑计划资助项目( 2011BA104B06)
浙江省重大科技攻关项目(2006C13014)
关键词
铁皮石斛
多糖
甘露糖
HPLC
D.officinale Kimura et Migo
Polysaccharide
Mannose
HPLC
作者简介
郑珊娇（1984-），女，浙江台州人，硕士研究生，研究方向：中药化学。
通讯作者：陈素红（1973-），女，浙江金华人，教授，硕士研究生导师，博士，研究方向：中药药理及产品开发。
