摘要
目的观察赤芍总苷(TPG)对人黑色素瘤A375细胞凋亡的影响并探讨其机制。方法将12.5、25、50、100、200 mg/L TPG分别加入体外培养的黑色素瘤细胞,对照组加等量生理盐水,孵育24、48 h后MTT测定细胞生长抑制率;25 mg/L TPG孵育细胞48 h,收集细胞,FCM测定细胞凋亡率;RT-PCR和Western blot分别测定Caspase-3、Bcl-2、Bax、Fas、FasL mRNA和蛋白的表达水平。结果 TPG抑制A375细胞生长作用明显,25 mg/L TPG孵育黑色素瘤细胞48 h后,细胞凋亡率增高,Caspase-3、Bax、Fas、FasL mRNA和蛋白表达也明显升高,Bcl-2 mRNA和蛋白表达水平下降,Bcl-2/Bax比值显著降低(均P<0.05)。结论 TPG可诱导黑色素瘤肿瘤细胞凋亡,其机制与上调Caspase-3、Fas和FasL表达水平,下调Bcl-2,降低Bcl-2/Bax比值密切相关。
Objective To investigate the influence of total paeonyglucosides（TPG）on cell apoptosis of human malignant melanoma A375 cell line and its mechanisms.Methods Human malignant melanoma cells were cultured with TPG（12.5,25,50,100,200 mg/L） for 24,48 h.Cell growth was detected by MTT.The cell apoptosis rate was obtained by FCM.Cells treated with 25 mg/L TPG for 48 h were collected and RT-PCR and Western blot assays were used to detect the mRNA and protein levels of Caspase-3,Bcl-2,Bax,Fas and FasL.Results TPG could inhibit the cell growth of A375.Compared with the control group,TPG significantly increased the cell apoptosis rate,and the expression of caspase-3,Bax,Fas,FasL and decreased the expression of Bcl-2 and Bcl-2/Bax（all P〈0.05）.Conclusions TPG induces cell apoptosis by up-regulating the caspase-3,Fas and FasL expression and decreasing the rate of Bcl-2 and Bax.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2011年第23期4606-4608,共3页
Chinese Journal of Gerontology
基金
河北省科技厅科研基金资助项目(10276105D-65)
关键词
赤芍总苷
黑色素瘤细胞株A375
凋亡
Total paeonyglucosides
Human malignant melanoma cell line A375
Apoptosis
作者简介
王亚珍（1972-），女，副主任护师，在读硕士，主要从事肿瘤中西医护理及教学工作。
通讯作者：吕品田（1975-），男，硕士，主治医师，主要从事晚期恶性肿瘤的中西医结合治疗研究。
