摘要
以国内4个大蒜栽培品种为材料,建立了以根为外植体的再生体系。将蒜瓣去皮后灭菌消毒,萌发后选取苗龄为5~7d的无菌苗的根接种到含不同激素配比的培养基中进行愈伤组织诱导,发现MS＋2,4-D1mg/L＋2ip0.1mg/L组合愈伤诱导效率最高,平均为56.06%;愈伤组织经过2~3次继代培养,选取胚性愈伤组织置于不同分化培养基上进行培养,2~3个月后可见小芽产生,分化培养基为MS＋KT1mg/L时,植株再生效率最高,平均达到35.01%。本研究建立了一种以根为外植体的高效的大蒜愈伤诱导和再生体系,为大蒜遗传转化体系的建立打下良好基础。
An efficient callus induction and plant regeneration system was developed in four garlic （Allium sativum. L） cultivars from China, using root segments from in vitro plantlets as explants. Cloves were peeled off and sterilized, then germinated in 1/2 MS medium. Roots from 5~7 d seedlings were inoculated onto different induction media to induce callus. MS media with 2,4 D 1 mg/L and 2ip 0.1 mg/L had the highest callus induction potential,and the average callus induction frequency was 56.06%. During the stage of plant regeneration,embryogenesis callus were prone to generate roots which restrained plant regeneration. MS with KT 1 mg/L had the highest shoot regeneration potential which was 35.01%. This system has potential to be used for garlic transformation.
出处
《生物技术进展》
2011年第2期140-145,F0003,共7页
Current Biotechnology
基金
转基因生物新品种培育重大专项--转基因新技术新方法研究(2008ZX08010-001)资助
关键词
大蒜
外植体
根
愈伤诱导
植株再生
garlic
explant
root
callus induction
plant regeneration
作者简介
唐巧玲，博士研究生，主要从事植物基因工程的研究。E-mail：aq0815@sina．com。
王志兴，研究员，博士生导师，主要从事生物安全研究、植物转化体系研究和植物抗病、抗虫、品质改良等基因工程研究。E-mail：wangcotton@126．com
