摘要
采用山西栽培的５个地方名蒜茎尖离体培养，获得了无病毒蒜种。结果表明，茎尖大小为０．２～０．５ｍｍ，不定芽增殖培养基以Ｂ５或ＭＳ．附加１～２ｍｇ／Ｌ细胞分裂素和０．０１～０．１ｍｇ／ＬＮＡＡ，效果最佳。根系诱导在原培养基中加入０．２ｍｇ／ＬＮＡＡ，出根率可达９０％以上。成苗后于１２月份移栽到节能日光温室，成活率达９０％以上。经对脱毒苗形态观察和染色体检测，没有发生异常变化，植株生长健壮，遗传性稳定，产量高。
Virus-free garlics were obtained by cultrring stem-tips of 5 of 5 famous local garlics cultivated in Shanxi. The results showed ththat when the media were MS or B5 supplemented with 1 -2mg/L BA and 0. 01 -0. 1ing/L NAA an,andehof zstem-tip eas 0. 2-0. 5mm, increase of regenerated buds was the most, and that the rratio of the induction of roots was more than 90% when the medium was MS or B5 containing.02mg/L NAA, T. Tsurvival tate was 90% or above after the regenerated plants were transplanted into the energy-saving daylight greenhouse.The results also proved that the virus-free garlic seedlings had not any abnormal changes. They could grow robustly and had a steady heredity and high production through investigating their shapes and examining their chromosomes.
出处
《华北农学报》
CSCD
北大核心
1995年第3期20-25,共6页
Acta Agriculturae Boreali-Sinica
关键词
大蒜
茎尖培养
脱毒
愈伤组织
Garlic
Stem-tip culture
Virus-free
Callus
