摘要
以T6代转抗旱相关基因GhDREB小麦株系‘G鲁麦23’及其受体对照‘鲁麦23’为材料,通过PEG-6000人工模拟干旱胁迫处理观察小麦萌发期胚芽鞘长度、胚根数、胚根长度,采用大田不同水分处理试验研究其不同生育时期的生理生化、形态指标与抗旱指数,以明确转基因材料的抗旱性.结果表明:(1)在种子萌发期20%(W/V)PEG-6000干旱胁迫条件下,‘G鲁麦23’及其受体对照‘鲁麦23’的胚芽鞘长度、胚根长度和胚根数均比非胁迫时降低,且‘G鲁麦23’的降幅较小但均显著高于受体对照;(2)与对照鲁麦23相比,‘G鲁麦23’具有较高的叶片相对含水量和可溶性蛋白含量;(3)随干旱胁迫程度的增强‘G鲁麦23’与‘鲁麦23’株高均有不同程度的降低,但G鲁麦23株高始终高于‘鲁麦23’;在不浇水条件下,‘G鲁麦23’穗叶距显著长于‘鲁麦23’;而3种水分处理下‘G鲁麦23’与‘鲁麦23’穗长几乎没有变化;(4)‘G鲁麦23’在不浇水和浇1次水条件下分别比对照增产11.68%和9.05%,其抗旱指数为1.22,属较强抗旱等级,表现出比‘鲁麦23’更强的抗旱、节水性能.
T6 offsprings of GhDREB gene ‘G Lumai 23’ and ‘Lumai 23’ were used as materials,their coleoptile length,radicle length and radicle numbers of wheat seedlings were investigated under PEG-6000 osmotic solution simulated drought stress,and their physiological and biochemical,morphological and drought resistance index were further analyzed under different water treatments at field case.The result indicated that comparison with normal,their coleoptile length,radicle length and radicle numbers were reduced under 20% concentrations PEG-6000 osmotic solution simulated drought stress,but the declining range of the control was greater than that of the transgenic lines;the relative water content,soluble protein of ‘G Lumai 23’ were observably higher than that of control plants;With the increasing of drought stress,the height of materials had different extent of decrease,but the transgenic lines always had higher plant height than that of control;The space of spike leaf of ‘G Lumai 23’ was significantly longer than that of ‘Lumai 23’ under W0 treatment（without irrigation）;The spike length almost have no change under three different irrigation levels.Under the W0 condition,the yield of ‘G Lumai 23’ exceed the control by 11.68%,and the drought index was 1.22,which belongs to a strong drought-resistant level.It is suggested that the transgenic lines ‘G Lumai 23’ had higher performance of drought resistance and water-saving.
出处
《西北植物学报》
CAS
CSCD
北大核心
2011年第7期1447-1452,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
转基因生物新品种培育重大专项重点课题(2009ZX08002-008B)
转基因生物新品种培育科技重大专项重大课题(2008ZX08002-002)
关键词
小麦
GhDREB基因
抗旱指标
抗旱性
wheat（Triticum aestivum L.）
GhDREB gene
drought index
drought resistance
作者简介
赵洁岚（1986-），女，在读硕士研究生，主要从事作物生物技术与育种研究。Email：zhjlclub@126．com
通讯作者：闵东红，博士，副研究员，主要从事小麦遗传育种研究。E—mail：mdh2493@126．com
