摘要
[Objective] This study aimed to evaluate the application potential of wheat MYB protein-encoding gene TaPIMP1 in the breeding of drought-tolerant wheat germplasm. [Method] The homozygous transgenic lines with TaPIMP1 gene which is driven by ubiquitin promoter and their recipient Yangmai 158 were cultivated under simulated drought condition. Then, seed germination, seedling growth, and some biochemical parameters associated with abiotic stress of the three cultivars were an- alyzed. [Result] In the medium containing 20% PEG6000, the expression level of TaPIMP1 gene in all the three wheat cultivars changed greatly within the first 24 h; the seed germination rate, the lengths of coleoptile and radical of two transgenic lines B64 and B208 were significantly higher than those of control Yangmai 158. In the medium containing 10%-20% PEG6000, the leaf relative water content and sol- uble sugar contents of two TaPIMP1 transgenic lines were significantly higher than those in control. In the medium containing 15%-20% PEG6000, the MDA content of two transgenic lines was significantly lower than that of the control. [Conclusion] The drought tolerance of the two transgenic lines has been improved obviously com- pared with Yangmai 158, so the TaPIMP1 gene can be introduced to develop new drought-tolerant wheat cultivars.
[目的]评价小麦TaPIMP1基因在小麦耐旱种质培育方面的应用潜力。[方法]将由ubiquitin启动子驱动的TaPIMP1转基因纯合株系B64和B208及受体对照扬麦158在模拟的干旱胁迫条件下进行培养，测定种子萌发、幼苗生长以及部分耐旱相关生化指标的变化。[结果]在20％PEG6000模拟干旱胁迫条件下，转基因株系及其对照的TaPIMP1表达量在24h内变化剧烈：转基因株系的种子发芽率、胚芽鞘和胚根长度显著高于受体对照。在10％-20％PEG6000模拟干旱胁迫条件下，转基因株系的叶片相对含水量和可溶性糖含量明显高于受体对照。在15％-20％PEG6000模拟干旱胁迫条件下。转基因株系的叶片丙二醛（MDA）含量明显低于受体对照。[结论]该研究表明转基因株系的耐旱能力较受体对照扬麦158有显著提高。TaPIMP1基因可应用于转基因小麦耐旱种质的培育。
基金
Supported by Key Special Project for Breeding and Cultivation of GMO Varieties(2013ZX08002001-004)
the Joint Innovation Fund of Jiangsu Province(BY2012208)
Agricultural Science and Technology Independent Innovation Fund of Jiangsu Province[CX132022]~~
作者简介
通讯作者：周森平（1968-），男，江苏兴化人，硕士，研究员，主要从事小麦生物技术育种研究，E—mail：mpzhou@hotmail．com.
