摘要
为快速高效提取作物基因组DNA,以南瓜品种为试材,对籽用南瓜基因组DNA的快速、高效提取方法进行研究,并对产物进行紫外分光光度计和琼脂糖凝胶电泳分析。结果表明:通过对CTAB改良后提取的基因组DNA结构较完整,杂质较少,浓度较高,可经纯化直接用于进一步的PCR等分子生物学研究。
In order to extract DNA fast and efficient,the seed squash was taken as materials to study the extraction method.It was analyzed by ultraviolet spectrophotometer and agarose gel electrophoresis.The results showed that structure of DNA was complete but had less impurities,its concentration was high,it could be directly used for the further study of molecular biology（such as PCR）.
出处
《黑龙江农业科学》
2011年第10期14-15,共2页
Heilongjiang Agricultural Sciences
作者简介
赵茜（1982-），女．内蒙古自治区乌兰察布市人，硕士，研究实习员，从事籽用南瓜病害及遗传育种研究。E—mail：zhaoqian0401@sina．com。
通讯作者：徐丽珍（1963-），女，黑龙江省双城市人，副研究员，从事籽用南瓜遗传育种及栽培技术研究。Email：jzsxulizhen@163．com。
