摘要
本项研究采用SDS法、CTAB法和高盐低pH法对辣椒(CapsicumannuumL.)叶片基因组DNA进行提取;紫外吸收检测法与琼脂糖凝胶电泳法对DNA的纯度进行检测。紫外吸收检测结果表明,SDS法提取的辣椒叶片DNA具有典型的天然DNA分子的标准紫外吸收光谱特点,其A260/A280在1.771～1.912之间。SDS法提取的DNA经琼脂糖凝胶电泳检测得到一条迁移率很低的整齐清晰的DNA谱带,所提取DNA的质量和产率均较高,用该法提取的辣椒DNA进行RAPD分析,DNA扩增效果较好,带形清晰、整齐,说明SDS法提取的DNA分子较为完整,能用作PCR模板来开展辣椒分子水平的研究。
SDS, CTAB and high salt ＆ low pH methods were used to extract DNA from hot pepper reaves in this experiment. UV-spectrophotometry and agarose gel electrophoresis were used to test DNA purity. The results of UV-spectrophotometry showed that DNA extracted with the SDS method had the typical absorption spectra and its ratio of absorption value （A260/A280） ranged from 1.771 to 1.912,it showed a consistent and clear DNA band of very low migration rate in the agarose gel electrophoresis and had higher purity and yield,and it was suitable for RAPD reaction. Electrophoresis results showed that its amplification was good, clear and uniform without trailing, indicating that DNA molecules extracted by SDS were more intact and could be used as templates in PCR for molecular biological research in hot pepper.
出处
《辣椒杂志》
2007年第4期39-43,共5页
Journal of China Capsicum
关键词
辣椒
DNA提取
紫外吸收检测
RAPD
hot pepper
DNA extraction
UV-spectrophotometry detection
RAPD
作者简介
俞文政（1978-），男，在读研究生，研究方向为作物遗传育种
