摘要
采用液氮－ＳＤＳ法、ＳＤＳ法和氯化苄法对辣椒的ＤＮＡ进行了提取。结果表明：液氮－ＳＤＳ法提取的ＤＮＡ具有典型的天然ＤＮＡ分子的标准紫外吸收光谱，其Ａ２６０／Ａ２８０在１．６０～１．７０之间，Ａ２６０／Ａ２３０在１．５～１．８之间，１００ｍｇ叶子ＤＮＡ产量为６０～９０μｇ。用该法提取的辣椒ＤＮＡ适于进行ＲＡＰＤ分析。ＳＤＳ法、氯化苄法不适于辣椒ＤＮＡ的提取。
The preparation of DNA samples with good quality is the basis for researches in plant molecular biology.The DNA samples was prepared from hot pepper variety“Baojialia”and sweet pepper variety“Qiemen”by liquid N2-SDS method,SDS method and Benzyl Chloride method,respectively.The results showed that DNA samples,obtained by liquid N2-SDS method,possessed the ultraviolet absorbance spectrum of the pure natural DNA,and the A260/A280 was 1.60～1.70,A260 /A230 was 1.5～1.8.The yield of 60～90μg DNA per 100mg young leaves was obtained by liquid N2-SDS method.Also,the DNA samples prepared by this procedure were suitable for RAPD analysis,those isolated by other methods were unfit.Some steps were modified in liquid N2-SDS method because the degradation of RNA in DNA samples could affect the RAPD patterns.
出处
《江西农业大学学报》
CAS
CSCD
1998年第2期180-183,共4页
Acta Agriculturae Universitatis Jiangxiensis
基金
热带作物生物技术国家重点实验室开放课题
