摘要
采用生物信息学的方法和工具对已在GenBank上注册的黑腹果蝇(Drosophila melanogaster)、致倦库蚊(Culex pipiens quinquefasciatus)、埃及伊蚊(Aedes aegypti)、意大利蜜蜂(Apis mellifera)、丽蝇蛹集金小蜂(Nasonia vitripen-nis)的雌激素相关受体ERR的核酸及氨基酸序列进行分析,并对其组成成分、理化性质、疏水性/亲水性、蛋白质二级及三级结构、分子系统进化关系等进行了预测和推断.结果表明:昆虫ERR是一个亲水性蛋白.在ERR整体蛋白结构中α-螺旋和不规则卷曲是ERR最大量的结构元件,而β-折叠则散布于整个蛋白质中.ERR蛋白的DNA结合区(DBD)主要由三个α-螺旋和三个β-折叠构成,包含两个锌指结构.配体结合区(LBD)主要由3个β-折叠和11个α-螺旋构成.
In the present study, estrogen- related receptors （ERR）from Drosophila melanogaster, Culex pipierm quinquefasciatus, Aedes aegypti, Nasonia vitripennis and Apis mellifera which were registered in Genbank, were analyzed and predicted by the tools of bioinformatics in the following aspects： the composition of nuclei acid sequences and amino acid sequences, protein physical and chemical properties, hydrophobicity or hydrophilicity, secondary and tertiary structures of protein, molecular phylogenetic evolution and so on. The results are as follows： ERR is a hydrophilic protein; the main motif of predicted secondary structure of ERR are alpha helix and random coil, beta turn and extended strand are spread in the whole secondary structure of protein;there are three β-strands and three α-helixs in DNA binding domain （DBD）, and three β - strands and eleven α-helixs in ligand binding domain （LBD）. The DBD is characterised by two Zinc-finger structures.
出处
《西北民族大学学报（自然科学版）》
2011年第2期73-80,90,共9页
Journal of Northwest Minzu University(Natural Science)
关键词
雌激素相关受体
黑腹果蝇
致倦库蚊
埃及伊蚊
意大利蜜蜂
丽蝇蛹集金小蜂
蛋白质结构预测
Estrogen-related receptor （ERR）
Drosophila melanogaster
Culex pipiens quinquefasciatus
Aedes aegypti
Apis mellifera
Nasonia vitripennis
Protein structure prediction
作者简介
欧阳霞辉（1978-），女，甘肃靖远人，副教授，博士，主要从事动物生殖与发育方面的研究．
