摘要
对新疆芥菜型油菜4个单、双低品系子叶柄再生频率的影响因素进行了研究，其中子叶柄日龄、6－BA、AgNO3、卡那霉素浓度及基因型等因素对不定芽的再生率有很大影响。试验结果表明：萌发4～5d的幼苗子叶柄再生频率最高；6－BA、AgNO3。对不定芽分化的促进效果在不同品系中有较大差异。通常浓度为4～6mg／L；不同品系对卡那霉素的耐受力有很大不同，其中新95－A在Kan15mg／L时仍有4．17％的绿苗率；再生芽生根较容易，移栽成活率较高。
In this paper, factors which influenced the high efficient regeneration system of four'singleor double low'strains of Xinjiang B. juncea were studied. Among those,the days of cotyledon petiole,con-centration of 6-BA,AgNO3, and Kanamycin, genotype, etc. played important roles- Results indicated: 4～5 days of cotyledon petiole had the highest regeneration rate; 6-BA and AgNO, had different effect amongfour strains, usually the best concentration was 4～6 mg/L；to these strains, the tolerance to kanamycin was different, strain Xin95-A still had 4. 17% green shoot rate when Kanamycin amount to 15 mg/L; root regeneration of those green shoots was easy,and the survival rate was high after transplanted.
出处
《西北农业学报》
CAS
CSCD
1999年第4期57-59,共3页
Acta Agriculturae Boreali-occidentalis Sinica
