摘要
目的观察稳消Ⅱ方对兔实验性颈动脉粥样硬化细胞间黏附分子-1(ICAM-1)及血管细胞黏附分子-1(VCAM-1)表达的影响,探讨其抗动脉粥样硬化的作用机制。方法将60只健康新西兰大白兔随机分为空白组、干预组(稳消Ⅱ方高、中、低剂量组)、辛伐他汀组、模型组。除空白组外,其余各组均采用高脂饲养及颈动脉内膜球囊损伤法建立兔颈动脉粥样硬化模型。稳消Ⅱ方干预8周后,采用ELISA法检测ICAM-1含量,免疫组化法观察VCAM-1表达。结果与空白组比较,各组ICAM-1、VCAM-1均显著上调(P<0.05);与模型组比较,干予组和辛伐他汀组指标均显著下调(P<0.05);与辛伐他汀组比较,稳消Ⅱ方低剂量组ICAM-1明显上调(P<0.05),余差异无统计学意义;干预组组间比较,高剂量组ICAM-1下调明显大于低剂量组(P<0.05),余差异无统计学意义。结论干预组可抑制ICVM-1、VCAM-1的表达,益于动脉粥样硬化斑块的防治与稳定,且与药物剂量之间存在一定的依赖关系。
Objective To observe the effect of different dose of Wenxiao Ⅱ Decoction on the expression of intercellular adhesion molecule-1（ICAM-1） and vascular cell adhesion molecule-1（VCAM-1） in experimental atherosclerosis rabbits,and explore its mechanism in treating atherosclerosis.Methods Sixty New Zealand rabbits were randomly divided into 6 groups：blank control,high-,medium-,low-dose group of Wenxiao Ⅱ Decoction,Simvastatin group and model control group.Carotid atherosclerosis was established by an operation,injured by balloon,and high cholesterol diet for 8 weeks until the mature atherosclerotic plaques formed.The rabbits were treated with respective drug for another 8 weeks.Then ICAM-1 was assayed by ELISA,and the expression of VCAM-1 was determined by immunohistochemistry staining.Results The expression of ICAM-1 and VCAM-1 in carotid atherosclerosis groups were significantly increased compared with blank control（P0.05）.ICAM-1 of low-dose group of Wenxiao Ⅱ Decoction was higher than that of Simvastatin group（P0.05）,but there were no difference between high-dose or medium-dose group of Wenxiao Ⅱ Decoction and Simvastatin group（P0.05）.Except for the expression of ICAM-1 in high-dose group of Wenxiao Ⅱ Decoction was lower than low-dose group（P0.05）,there were no significant differences in groups of Wenxiao Ⅱ Decoction neither ICAM-1 nor VCAM-1（P0.05）.Conelusion Different dose of Wenxiao Ⅱ Decoction can inhibit the expression of ICAM-1 and VCAM-1,which may stabilize plaque of atheroselerosis,and there was direct correlation between therapeutic effect and dosage.
出处
《中国中医药信息杂志》
CAS
CSCD
2011年第7期43-45,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
上海市卫生局中医药科研基金(2008J003A)
上海市第六人民医院资助项目(200797)
关键词
稳消Ⅱ方
动脉粥样硬化
细胞间黏附分子-1
血管细胞黏附分子-1
兔
Wenxiao Ⅱ Decoction
atherosclerosis
intercellular adhesion molecule-1
vascular cell adhesion molecule-1
rabbits
作者简介
通讯作者：霍清萍，E-mail：huoqingping005@yahoo.com．cn
