摘要
目的研究导致半夏干燥过程中发生褐变的机制。方法采用紫外分光光度法检测干燥过程中半夏中多酚氧化酶活性的变化,使用色彩色差计测量半夏表面颜色的变化值,考察酶促反应是否是发生褐变的唯一机制。采用HPLC-ELSD法检测半夏中还原性糖的种类及含量,分析半夏中的氨基酸种类,判断其发生羰氨反应的可能性。结果导致半夏褐变的反应并不唯一,酶促反应对其颜色变化的影响主要发生在干燥前期,羰氨反应对半夏褐变也有影响。结论导致半夏干燥过程中发生褐变的机制并不单一,酶促反应和羰氨反应均对其颜色变化有影响。
Objective To study the browning mechanism of drying Pinelliae Rhizoma.Methods The activity of polyphenol oxidase（PPO） was detected by ultraviolet spectrophotometry,and the surface color data of drying Pinelliae Rhizoma were measured by chromaticity instrument.To draw a conclusion that whether Enzymatic browning was the unique factor of browning reaction or not by analyzing the relationship between the above two sets of data.To detect the kinds and content of reducing sugar by HPLC-ELSD and measure the kinds of amino acids in Pinelliae Rhizoma by amino acid analyzer.Through testing the results the possibility of Maillard reaction was analyzed.Results The factor of the color-change of Pinelliae Rhizoma is not unique,Enzymatic browning is in the prophase of the drying process and Maillard reaction also contributes to the color-change.Conclusion The factor in the process of Pinelliae Rhizoma color-change is not single.Both enzymatic browning and Maillard reaction impact on the color-change of Pinelliae Rhizoma.
出处
《中草药》
CAS
CSCD
北大核心
2011年第5期877-880,共4页
Chinese Traditional and Herbal Drugs
基金
四川省教育厅重点项目(08ZA119)
四川省科技厅科技支持计划(2009sz0110)
作者简介
严茂伟，男，山东泰安人，成都中医药大学在读硕士生，研究方向为中药新制剂和新剂型的研究。E-mail：279019166@qq．com
通讯作者孙灵根Tel：（028）61801001E—mail：wcj-one@263．net
