摘要
Using in vitro leaves of Zizyphus jujuba ‘Kongfusucui’ plantlet as explants,effects of culture method,phytohormone proportion and culture time on induction rate of adventitious shoot from leaves and effects of sucrose concentrations in rooting media on rooting of adventitious shoot were studied.The results show that induction rate of adventitious shoot by two-step culture method(firstly cultured on medium Ⅰ for four weeks then cultured on medium Ⅱ for three weeks) is significantly higher than that by one-step culture method(cultured continuously on medium Ⅰ for seven weeks).Phytohormone proportion in medium Ⅰ has an obviously influence on induction rate of adventitious shoot,which gradually increases with increasing of TDZ concentration in medium Ⅰ.And adding 1.0 mg·L-1 TDZ in medium Ⅰ leads to induction rate with above 80%.With culture time prolonging(cultured for 1,2,3 or 4 weeks on medium Ⅰ,respectively),induction rate increases gradually.Sucrose concentration has a significant effect on rooting of adventitious shoot,sucrose with higher concentration(30 g·L-1) is beneficial to rooting.According to induction rate and growth status of adventitious shoot,it is determined that optimal culture method of adventitious shoot induction from Z.jujuba 'Kongfusucui' leaves is two-step culture method,that is,leaves firstly cultured on WPM medium containing 1.0 mg·L-1 TDZ and 0.5 mg·L-1 IAA for four weeks,and then cultured on WPM medium containing 0.5 mg·L-1 IAA and 1.0 mg·L-1 GA3 till adventitious shoot produced.
Using in vitro leaves of Zizyphus jujuba ＇Kongfusucui＇ plantlet as explants,effects of culture method,phytohormone proportion and culture time on induction rate of adventitious shoot from leaves and effects of sucrose concentrations in rooting media on rooting of adventitious shoot were studied.The results show that induction rate of adventitious shoot by two-step culture method（firstly cultured on medium Ⅰ for four weeks then cultured on medium Ⅱ for three weeks） is significantly higher than that by one-step culture method（cultured continuously on medium Ⅰ for seven weeks）.Phytohormone proportion in medium Ⅰ has an obviously influence on induction rate of adventitious shoot,which gradually increases with increasing of TDZ concentration in medium Ⅰ.And adding 1.0 mg·L-1 TDZ in medium Ⅰ leads to induction rate with above 80%.With culture time prolonging（cultured for 1,2,3 or 4 weeks on medium Ⅰ,respectively）,induction rate increases gradually.Sucrose concentration has a significant effect on rooting of adventitious shoot,sucrose with higher concentration（30 g·L-1） is beneficial to rooting.According to induction rate and growth status of adventitious shoot,it is determined that optimal culture method of adventitious shoot induction from Z.jujuba ‘Kongfusucui’ leaves is two-step culture method,that is,leaves firstly cultured on WPM medium containing 1.0 mg·L-1 TDZ and 0.5 mg·L-1 IAA for four weeks,and then cultured on WPM medium containing 0.5 mg·L-1 IAA and 1.0 mg·L-1 GA3 till adventitious shoot produced.
出处
《植物资源与环境学报》
CAS
CSCD
2011年第1期83-85,共3页
Journal of Plant Resources and Environment
基金
国家林业科技支撑计划项目(2008BAD92B03)
山东省农业良种工程项目(2008LZ010和2009LZ010)
山东省农业科学院博士科研启动基金项目(2007YBS004)
泰安市科技发展计划项目(20093021)
关键词
'孔府酥脆’枣
离体叶片
不定梢诱导
植株再生
两步培养法
Zizyphus jujuba ‘Kongfusucui’
in vitro leaf
adventitious shoot induction
plantlet regeneration
two-step culture method
作者简介
通信作者孙清荣（1963-），女，山东无棣人，博士，研究员，主要从事果树生物技术育种研究。E-mail：sunqr@sdip．cn
