摘要
以酸枣无菌苗叶片为外植体,研究了培养条件对不定梢再生及不定梢玻璃化的影响.结果表明,叶片在加有细胞分裂素TDZ的诱导培养基(培养基Ⅰ)上连续培养,可诱导不定芽形成,但不能进一步发育成不定梢;而在诱导培养基Ⅰ上培养2周后转移到不加TDZ的培养基Ⅱ上,可获得不定芽伸长的不定梢.培养基Ⅱ的基本培养基组成影响不定芽(梢)的玻璃化症状:MS培养基产生玻璃化的不定芽(梢),而WPM培养基产生正常不定芽梢;光培养条件的变化对玻璃化症状的发生没有影响.不定芽(梢)玻璃化的发生可能与培养基中铵或硝酸铵的浓度有关,在不定芽伸长发育阶段,培养基中高浓度的铵导致了玻璃化苗的发生.
In vitro leaves of sour jujube（Zizyphus spinosus Hu.） were selected as explants.Effects of culturing conditions on adventitious bud formation and hyperhydricity were investigated.When leaf explants were cultured on medium containing TDZ（medium Ⅰ） continuously,adventitious buds were obtained but failed to develop into shoots.When leaf explants were cultured on medium Ⅰ for 2 weeks,then subcultured on the medium without TDZ（medium Ⅱ）,adventitious buds were obtained and developed into shoots.The formulation of basal medium of medium Ⅱ affected hyperhydricity of adventitious buds or shoots.The adventitious buds or shoots showed symptom of hyperhydricity when medium Ⅱ was MS and showed normal when medium Ⅱ was WPM.The dark culture had no effect on hyperhydricity.Hyperhydricity may be related to the increase of ammonium nitrate in MS medium since higher ammonium concentration in the medium at the stage of adventitious bud elongation resulted in adventitious shoots hyperhydricity.
出处
《西北植物学报》
CAS
CSCD
北大核心
2010年第5期1039-1044,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家林业科技支撑计划(2008BAD92B03)
山东省农业良种工程项目(2005LZ07-01
2006LZ07-01
2008LZ010)
山东省农业科学院博士科研启动基金(2007YBS004)
泰安市科技发展计划(20093021)
关键词
酸枣
叶片
不定芽
不定梢
玻璃化
sour jujube
leaf explants
adventitious bud
adventitious shoot
hyperhydricity
作者简介
孙清荣（1963-），女（汉族），博士，研究员，主要从事果树生物技术育种研究。Email：sunqr@sdip．cn.
