摘要
应用分子印迹的方法制备对地高辛有特异性吸附性能的印迹聚合物颗粒,再将颗粒与琼脂糖混合并固定于玻碳电极上制备成地高辛分子印迹聚合膜传感器,传感器可以特异性地结合模板分子地高辛且其电化学信号与模板浓度相关,再用它来检测血清中地高辛的含量。结果表明:分子印迹传感器具有制作简便、成本低、检测快速、特异性高、稳定性好等优点,检测下限为1.28 nmol/L,检测时间为5 min。
The biosensor was prepared by molecular imprinted technique with digoxin-specific adsorption properties.Firstly the imprinted polymer particles were prepared.Then,particles and the agarose were mixed in proportion.The molecular imprinted polymer film sensor for the digoxin was fixed by the mixture on a glassy carbon electrode（GC）.The sensor had specific combination of template molecule-digoxin and its electrochemical signals associated with the template concentration.Thus,it could be used to detect digoxin levels in serum.The molecular imprinted biosensor had advantages of simple,low cost,rapid detection,high specificity,and good stability.The detection limit of the biosensor was 1.28 nmol/L and detection time was 5 min.
出处
《生物加工过程》
CAS
CSCD
2011年第2期64-67,共4页
Chinese Journal of Bioprocess Engineering
基金
科技部资助项目(2009GJA10047)
天津市科委资助项目(08ZXCXSH053
09ZCZDSF04200)
作者简介
李敏（1985-），女，内蒙古包头人，硕士研究生，研究方向：电化学生物传感器；
张娟琨（联系人），教授，博士生导师，E-mail：zhangjk@rust．edu．cn
