摘要
本文构建了能够快速检测邻苯二甲酸二异壬酯(DINP)的分子印迹电化学传感器.首先利用分子印迹技术以热聚合的方法制备了分子印迹聚合物颗粒(MIPs);然后将优化的MIPs与琼脂糖溶液混匀固定在玻碳电极表面制备成DINP分子印迹电化学传感器;传感器可以特异性结合模板分子DINP且其电化学信号与DINP的浓度相关.再利用传感器检测样品中DINP的含量,检测过程不需要样本前处理,可以用于现场直接检测.该方法的线性方程为ΔI=0.03c+3.07,相关系数R^2=0.998,线性范围5×10^(-8)~1×10^(-6),mol/L,检测限为2.7×10^(-8),mol/L,在实际样品中的加标回收率为105.3%,~115.7%,.
An electrochemical sensor based on molecularly imprinted sensor for quick detection of DINP was developed in this research.Firstly,imprinted polymer particles(MIPs)were prepared through thermal polymerization.Then,MIPs and agarose were mixed in proportion.The electrochemical sensor for DINP was fixed by the mixture on a glassy carbon electrode.The sensor can be combined with the template molecule DINP and its electrochemical signals are influenced by DINP concentration.Thus,it could be used to detect DINP levels in samples,and DINP concentration was quickly detected in wine samples without sample pretreatment.The linear equation in this study was ΔI=0.03c+3.07,the correlation coefficient is R2=0.998,the linear range was 5×10-8-1×10-6,mol/L,the sensitivity limit was 2.7×10-8,mol/L,and the recovery rate was 105.3%,-115.7%,.
作者
陶强
张娟琨
刘英姿
鞠小翠
左娟娟
TAO Qiang;ZHANG Juankun;LIU Yingzi;JU Xiaocui;ZUO Juanjuan(Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,Tianjin Key Laboratory of Industrial Microbiology,College of Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China)
出处
《天津科技大学学报》
CAS
北大核心
2016年第5期14-18,共5页
Journal of Tianjin University of Science & Technology
基金
国际合作项目(14RCCFSF00140)
关键词
邻苯二甲酸二异壬酯
分子印迹聚合物颗粒
电化学传感器
检测
循环伏安
diisononyl phthalate
molecularly imprinted polymer particles
electrochemical sensor
detection
cyclic voltammetry
作者简介
陶强（1990—），男，河南人，硕士研究生;通信作者：张娟琨，教授，zhangjk@tust.edu.cn.
