摘要
从土壤中筛选出了一株产葡萄糖氧化酶较高的菌株,利用响应面法对该菌株的产酶培养基进行优化以提高产酶量。响应面法优化的发酵培养基组成为:葡萄糖109.41g/L、复合氮源(m(月示蛋白胨):m((NH4)2SO4)=3:1)37.36g/L、吐温-80 37.15g/L、KH2PO4 2g/L、MgSO4.7H2O 0.7g/L和KCl 0.5g/L。采用该优化培养基所得葡萄糖氧化酶的活力为1.54U/mL,较优化前提高了31.6%。
Glucose oxidase（GOD） has gained considerable commercial importance due to its multitude of applications in the food,pharmaceutical,biotechnology and other industries.In the present investigation,a high GOC-producing strain was isolated from the soil of Wenwu Xuefeng farm in Fuzhou,and the fermentation medium was optimized by response surface methodology for higher GOD production by the isolated strain.The optimal medium composition determined consisted of glucose 109.41 g/L,complex nitrogen source（proteose peptone ＋（NH4）2SO4,3：1,m/m） 37.36 g/L,Tween-80 37.15 g/L,MgSO4·7H2O 0.7 g/L,KH2PO4 2 g/L and KCl 0.5 g/L.A maximum GOD enzyme activity of 1.54 U/mL was obtained using the optimized fermentation medium,31.6% higher than before optimization.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第1期149-153,共5页
Food Science
关键词
葡萄糖氧化酶
酶活力
培养基优化
响应面
glucose oxidase
enzyme activity
medium optimization
response surface methodology
作者简介
韩建春（1973-），男，副教授，博士，研究方向为畜产品加工。E-mail：hanjianchun@hotmail．com
