摘要
对一株具有产γ-氨基丁酸(γ-aminobutyric acid,GABA)能力的植物乳杆菌(Lactobacillus plantarumMJ0301)进行发酵培养基成分优化,以提高该菌产GABA能力。通过单因素试验考察碳源、氮源、K2HPO4、吐温-80和L-谷氨酸钠(monosodiumL-glutamate,L-MSG)对L.plantarumMJ0301生长及GABA产量的影响。结果表明,葡萄糖、氮源(胰蛋白胨和酵母膏)和L-谷氨酸钠3个因素对L.plantarumMJ0301生长和GABA产量影响较大。采用Box-Behnken中心组合试验设计原理,选定葡萄糖、复合氮源(胰蛋白胨和酵母膏)和L-谷氨酸钠为三因素三水平进行响应面试验,以GABA产量为响应值,在分析各个因素的显著性和交互作用后,确定L.plantarumMJ0301产GABA的最佳培养基成分,即葡萄糖16.0g/L、氮源10g/L、L-谷氨酸钠30g/L、柠檬酸三胺2g/L、K2HPO42g/L、乙酸钠5g/L、硫酸镁0.1g/L、硫酸锰0.05g/L、吐温-801mL/L。此时,L.plantarumMJ0301产GABA的量由原来的143mg/L增加到1.59g/L,提高了10.12倍。
The aim of the present study was to investigate the optimization of formulation of culture medium for γ-aminobutyric acid （GABA）-producing lactic acid bacteria. The effects of carbon sources, nitrogen sources, K2HPO4, Tween-80 and monosodium L-glutamate （L-MSG） on the growth of L. plantarum MJ0301 and the yield of GABA were explored by single factor test. The results showed that glucose, nitrogen （tryptone and yeast extract） and L-MSG significantly influenced the growth of L. plantarum MJ0301 and the yield of GABA （P 〈 0.05）. The response surface methodology was designed based on glucose, nitrogen （tryptone and yeast extract） and L-MSG, and the yield of GABA was considered as response value. The optimal formulation of the culture medium consisted of glucose 16.0 g/L, mixed nitrogen sources 10 g/L, L-MSG 30 g/L, ammonium citrate 2 g/L, K2HPO4 2 g/L, sodium acetate 5 g/L, magnesium sulfate 0.1 g/L, manganese sulfate 0.05 g/L, and Tween-80 1 mL/L. The resulting yield of GABA was 1.59 g/L, which was 11.12-fold higher than before the optimization （143 mg/L）.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第17期165-170,共6页
Food Science
基金
江南大学自主科研计划项目(1022050205123950)
作者简介
黄桂东（1978-），女，副教授，博士，主要从事食品生物技术研究。E—mail．guidongh78@126．com
通信作者：毛健（1970-），男，教授，博士，主要从事食品生物技术研究。E—mail：maojiand417@163．com
