摘要
采用末端限制性片段长度多态性(T-RFLP)技术研究地黄连作下根际土壤细菌群落的动态变化.结果表明:地黄根际土壤细菌群落的香农多样性指数、丰富度指数和相似性指数均为对照(CK)>1年>2年,地黄连作下细菌优势种群的比例显著下降,种植1年土壤中厚壁菌门的芽孢杆菌纲在整个细菌群落中处于主导地位,而种植2年土壤中变形菌门ε-变形菌纲处于主导地位.地黄连作使其根际土壤细菌种类大量减少,群落结构趋于简单.连作后细菌群落多样性水平的变化导致根际土壤微生物群落功能的失调,可能是引发连作障碍的原因之一.
In this paper,T-RFLP（terminal restriction fragment length polymorphism） technique was adopted to study the dynamic changes of bacterial community in the rhizosphere soil of continuously cropped Rehmannia glutinosa L.The results showed that the Shannon diversity index,Margalef index,and similarity index of bacterial community in the rhizosphere soil all decreased in the order of control one-year cropping two-year continuous cropping.Under continuous cropping,the proportion of dominant bacterial species declined obviously.In one-year cropping soil,the class Bacilli of phylum Firmicute dominated the bacteria community;while in two-year continuous cropping soil,the class Epsilonproteobacteria of phylum Proteobacteria became dominant.Continuous cropping of R.glutinosa decreased the bacteria species,and simplified the bacterial community structure.The changes of bacterial community diversity under continuous cropping of R.glutinosa led to the disorder of the functions of bacterial community,and thereby,the destruction of the ecological balance in rhizosphere soil,which might be one of reasons causing the obstacles of continuous cropping of R.glutinosa.
出处
《应用生态学报》
CAS
CSCD
北大核心
2010年第11期2843-2848,共6页
Chinese Journal of Applied Ecology
基金
国家自然科学基金项目(30772729
30671201)
国家"十一五"科技支撑计划项目(2006BAI09B03)资助
作者简介
通讯作者．E-mail：wenxiong181@163．com
