摘要
以‘红福七寸’和‘红芯七寸’胡萝卜为材料,测定了不同浓度Na Cl处理下肉质根木质素含量以及根和叶中胁迫相关的生理指标,并利用实时荧光定量PCR方法测定了13个木质素合成相关基因在肉质根中的表达水平。结果显示:在不同浓度盐胁迫下,胡萝卜肉质根中木质素含量均显著高于对照(蒸馏水处理),且在100 mmol·L-1Na Cl处理下含量最高,为对照的1.91倍(‘红福七寸’)和1.83倍(‘红芯七寸’),表明盐胁迫诱导了胡萝卜肉质根木质素的合成。q RT-PCR和相关性分析结果显示13个木质素合成相关基因中的Dc F5H和Dc COMT表达水平与木质素含量的动态变化相似且高度正相关,可能为盐胁迫下胡萝卜肉质根木质素生物合成响应的关键基因。生理指标测定结果显示,在盐胁迫处理下,胡萝卜根和叶中的超氧化物歧化酶(SOD)活性、脯氨酸和可溶性蛋白含量均有不同程度的响应。本研究结果表明盐胁迫诱导胡萝卜肉质根的木质化,木质素合成通路基因参与了对盐胁迫的响应,Dc F5H和Dc COMT可能是盐胁迫下调控木质素合成的关键基因。
‘Hongfu Qicun’and‘Hongxin Qicun’carrots were used as materials to detect the lignin contents in taproot.Stress-related physiological parameters in taproots and leaves under different Na Cl concentration treatments were also determined.The expression levels of 13 lignin biosynthesis related genes in taproot were detected by real-time fluorescence quantitative PCR method.The results showed that the lignin contents of carrot taproot under different salt stress treatments were significantly higher than that of the control(distilled water treatment).The lignin contents were the highest under 100 mmol·L-1 Na Cl treatment,which were 1.91 times(‘Hongfu Qicun’)and 1.83 times(‘Hongxin Qicun’)of the control,respectively.The results indicated that salt stress induced the biosynthesis of lignin in carrot taproot.The q RT-PCR and correlation analysis results showed that the Dc F5 H and Dc COMT expression levels of 13 lignin-related genes were similar and high-positively correlated with the dynamic changes of lignin content.The two genes may be the key lignin biosynthesis related genes of carrot taproot in response to salt stress.The physiological indexes demonstrated that the superoxide dismutase(SOD)activity,proline and soluble protein contents in carrot taproots and leaves responded to salt stress treatments.The results of this study indicated that salt stress induced the lignification of carrot taproot,and lignin biosynthesis pathway genes were involved in response to salt stress.Dc F5 H and Dc COMT may be the key regulating genes of lignin biosynthesis under salt stress in carrot taproot.
作者
陈皓炜
陈梦娇
王雅慧
张榕蓉
王欣蕊
徐志胜
谭国飞
熊爱生
CHEN Haowei;CHEN Mengjiao;WANG Yahui;ZHANG Rongrong;WANG Xinrui;XU Zhisheng;TAN Guofei;XIONG Aisheng(College of Horticulture,State Key Laboratory of Crop Genetics and Germplasm Enhancement,Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China,Nanjing Agricultural University,Nanjing 210095,China;Institute of Horticulture,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2021年第1期153-161,共9页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31872098)
江苏高校优势学科建设项目(PAPD)。
关键词
胡萝卜
盐胁迫
木质素
基因表达
生理特性
carrot
salt stress
lignin
gene expression
physiological characteristics
作者简介
通信作者:熊爱生,E-mail:xiongaisheng@njau.edu.cn。
