摘要
旨在研究并比较DL-α-生育酚醋酸酯（DL-α-TOA）及不同水平D-α-生育酚琥珀酸酯（D-α-TOS）日粮对肉鸡的生长、抗氧化功能与肉品质的影响。选用1日龄Arbor Acres（AA）肉鸡320只，随机分为4个处理，分别在基础日粮中添加30mg·kg-1 DL-α-TOA（对照组）及D-α-TOS10mg·kg-1（α-TOS1组）、30mg·kg-1（α-TOS2组）和50mg·kg-1（α-TOS3组），试验期42d。结果表明：对照组及各α-TOS组间肉鸡的生长均无显著差异（P〉0．05）；相关性分析结果表明，肉鸡日粮中D-α-TOS的添加水平与血清（R2=0．9831，P〈0．01）和肝脏（R2=0．9336，P〈0．05）中α-生育酚（α-TOH）的含量呈显著正相关，与血清（R2=-0．9487，P〈0．05）和肝脏（R2=-0．9901，P=0．0518）丙二醛（MDA）呈负相关；与对照组和a—TOSl组相比，日粮中添加30～50mg·kg α-TOS能够使血清总超氧化物歧化酶（T—SOD）、谷胱甘肽过氧化物酶（GSH—Px）、总抗氧化能力（T—AOC）和肝脏的T—SOD活性及T-AOC均有不同程度地提高（P〈0．05或P〈0．01），同时肝脏活性氧（ROS）水平也显著降低（P〈0．05）；在肉品方面，日粮中添加30～50mg·kg-1 α-TOS显著降低了肉鸡胸腿肌的滴水损失（48h）和剪切力，及腿肌的烹饪损失，但对pH45min、pH24h、24h滴水损失和肉色无显著影响（P〉0．05）。结果提示，日粮中添加30～50mg·kg-1 D-α-TOS能够在一定程度上提高肉鸡机体的抗氧化功能，提高了肌肉的系水力和嫩度，其原因主要是α-TOS的添加导致rTOH在血清和肝脏中含量的提高，及肝脏MDA和ROS水平的降低。
The objective of this study was to compare the effect of two esters of α-tocopherol, all- rac-α-tocopherol acetate （DL-α-TOA） and RRR-α-tocopherol succinate （D-α-TOS） on growth, antioxidant capacity and meat quality in broilers. 320 one-day-old commercial Arbor Acres broilers were randomly distributed into 4 treatments, each of which had 8 pens of 10 chicks per pen. Birds were fed with the diets supplemented with 30 mg·kg-1 DL-α-TOA （control）, the basal diet with D-α-TOS supplementation 10 mg·kg-1 （TOS1 group）, 30 mg·kg-1 （TOS2 group） and 50 mg·kg-1 （TOS3 group）, respectively for 42 d. The results showed that there was no significant difference （P;〉0.05） in the growth among the treatments. Significant positive correlations existed between dietary supplemental a-TOS levels and plasma （R2= 0. 983 1, P〈0.01） or hepatic （R2=0. 933 6, P〈0.05）α-tocopherol concentrations, and a negative correlation with plasma （R2=0. 948 7, P〈0.05） or hepatic （R2=-0. 990 1, P〈0.051 8） malondialdehyde（MDA） levels. In comparison with the control and α-TOS1 groups, 30-50 mg·kg-1 dietary α-TOS supplementation resulted in an increase in the activities of serum T-SOD, GSH-Px, T-AOC and hepatic T-SOD, T-AOC to some extent （P〈0.05 or P〈0.01）. Furthermore, the level of hepatic ROS was decreased significantly （P〈0.05）. As for the meat quality, 48 h drip loss and shear force of breast and leg muscle was significantly decreased in broilers of TOS2 and TOS3 groups and also the cooking loss of leg muscle. But no significant differences were observed on pH45min, pH24h, 24 h drip loss and meat color （P〉0.05）. These results indicated that 30-50 mg·kg-1 dietary D-α-TOS supplementation could enhance the antioxidant capacity of broilers, and further the water holding capacity and tenderness, which might result from increased retention of serum and hepatic α-tocopherol and reduction in lipid peroxidation, as evidenced by the de- crease in MDA and ROS.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2010年第3期286-294,共9页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家重点基础研究发展计划(973)(2004CB117500)
关键词
α-生育酚琥珀酸酯
肉鸡
抗氧化酶
肉品质
RRR-α-tocopherol succinate
broiler
antioxidant enzyme
meat quality
作者简介
张旭晖（1982-），女，山东平度人，博士生，主要从事动物营养与饲料科学研究，Tel：025—84396483，Email：huihui19820131@163．com
通讯作者：王恬，E-mail：twang18@163．com
