摘要
以蚕蛹为主要原料,通过对米曲霉的培养筛选,确定了沪酿3.042是最佳的菌种。研究了培养时间、温度、曲料含水量、碳源、碳源添加量和接种量对制曲的影响。结果表明,当培养时间72h,培养温度30℃,曲料含水量40%,接种量为0.5%,以葡萄糖为主要碳源时,米曲霉产中性蛋白酶为2135U/g,孢子数为1.92×109个/g,氨基酸态氮为1.212g/100g。制曲后蚕蛹的风味得到极大改善。
Using the silkworm pupa as the main material, strain AS 3.042 was confirmed to be the optimum stain by culturing and screening different Aspergillus oryzaes. The optimal conditions were obtained as follows： the fermentation time 72 h, the fermentation temperature 30℃, the mois content of substrate 40%, the inoculum 0.5% and main carbon source glucose. Under these conditions, the maximum neutral protease activity were 2135U/g, respectively. The number of spores was 1.92×10^9cfu/g and the content of amino-acid nitrogen was 1.212 g/100g. Moreover, the flavor of silkworm pupa was greatly improved.
出处
《中国酿造》
CAS
北大核心
2010年第3期106-109,共4页
China Brewing
基金
辽宁省科学技术计划项目(2008205002)
关键词
蚕蛹
制曲
蛋白酶活力
氨基酸态氮
silkworm pupa
koji-making
proteinase activity
amino-acid nitrogen
作者简介
孔繁东（1958-），男，教授，研究方向为蛋白质资源开发利用。
