摘要
采用水培方法,在玉米幼苗进入三叶期后向培养液中加入不同浓度氯化镉(0、10-6、10-5、10-4mol·L-1),胁迫不同时间(5、10、15d)后对幼苗进行各项指标检测。结果表明,低浓度(10-6mol·L-1)及中浓度(10-5mol·L-1)镉胁迫对幼苗生长均有较强刺激作用,而中浓度胁迫对地上部分作用更明显;低浓度镉胁迫对根部及叶片抗氧化酶(SOD、POD、CAT及ASA-POD)活性影响较小,只是根部POD、ASA-POD活性明显升高,而中浓度胁迫对除SOD外其他抗氧化酶活性均有显著促进作用;MDA含量均未有显著升高,可见根部及叶片未受到氧化损伤;两种浓度胁迫下,Fv′/Fm′虽下降,净光合速率有所升高。高浓度镉(10-4mol·L-1)胁迫下幼苗生长几乎停止;根部SOD及CAT活性升高,而POD和ASA-POD被明显抑制,叶片中CAT、POD及ASA-POD活性显著升高;根部受到显著氧化损伤;各项光合参数(Fv/Fm、Fv′/Fm′和净光合速率)均显著降低。
The influence of Cd^2＋ to the antioxidant system and photosynthesis of maize（ Zea mays L. ） was studied systematically. Seedlings were cultivated in Hoagland liquid medium, and in the three-leave-period were treated with different concentrations of CdC12 （0, 10^-6, 10^-5, 10^-4 mol· L^-1 ）. After 5,10 and 15 days of stress, physiological parameters of seedlings were measured. In the stress of low（ 10^-6 mol· L^-1 ） and middle（ 10^-5 mol·L^-1 ） concentration, the growth of seedlings was promoted obviously whereas that of parts above the earth more notably in the stress of 10^-5 mol·L^-1; In the stress of 10^-6 mol·L^-1, the activities of POD and ASA-POD in root increased and activities of other anti-oxidant enzymes changed little; However, the stress of middle concentration made activities of anti-oxidant enzymes increase obviously except SOD; The content of MDA was not high compared with control, which showed that seedlings were not hurt by oxidation; Fv′/Fm′ decreased and the net photosynthetic rate rose. In the concentration of 10^-4 mol· L^-1, the seedlings almost did not grow; The activities of SOD and CAT in root increased and those of POD and ASA-POD were inhibited seriously, and the activities of CAT, POD and ASA-POD in leave increased sharply; Root was hurt oxidately; Photosynthetic parameters（Fv/Fm, Fv ′/Fm′ and net photosynthetic rate ） decreased notably.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2010年第2期211-215,共5页
Journal of Agro-Environment Science
基金
天津师范大学博士启动基金(5RL034)
国家自然科学基金(30570101)
关键词
镉
玉米
抗氧化酶
丙二醛
叶绿素荧光参数
cadmium
Zea mays
antioxidant enzyme
MDA
parameters of chlorophyll fluorescence
作者简介
黄辉（1976-），男，天津人，博士，副教授，主要从事植物生物化学和光合作用方面研究。E-mail：hippoh@sina．com．cn
