摘要
采用纤维素酶和果胶酶提取柳蒿芽中的多糖,通过单因素试验和正交试验分析了料液比、时间、酶加量、温度、pH值5个因素对提取柳蒿芽多糖的影响。结果表明:酶法提取多糖的适宜条件是纤维素酶为底物质量的0.5%、果胶酶为底物质量的2.0%,加热时间为0.5h,温度55℃,溶液pH值为5.5,料液比(m(柳蒿芽):V(提取液))为1g:20mL,此时的提取率为5.37%。
An experiment was conducted to extract polysaccharide from Artemisia integrifolia by cellulase and pectase. The optimal parameters for the extraction technology were determined by single factor and orthogonal tests. The optimal extraction process was obtained as cellulase 0.5% , pectase 2.0% , extraction time 0.5 hour, temperature 55 degrees C, pH 5.5, and liquid to material ratio 1 g： 20 mL. The extraction rate of polysaccharide was 5.37% under this condition.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2009年第12期54-55,共2页
Journal of Northeast Forestry University
基金
东北林业大学本科生创新实验基金
关键词
柳蒿芽
多糖提取
工艺条件
纤维素酶
果胶酶
Artemisia integrifolia
Polysaccharide extraction
Process conditions
Celhdase
Pectase
作者简介
第一作者简介：朱翘楚，女，1987年11月生，东北林业大学林学院，本科生。
