摘要
目的:建立测定鸡骨草药材中相思子碱和下箴刺桐碱含量的方法。方法:采用反相高效液相色谱法,色谱柱为KromasilC18(250mm×4.6mm,5μm),流动相为水-甲醇-乙腈-冰醋酸-三乙胺(82∶13∶5∶0.2∶0.3),流速为1.0mL·min-1,检测波长为220nm,柱温为室温。结果:相思子碱和下箴刺桐碱线性范围均为1.1～55mg·mL-1(r=0.9999);平均回收率(n=6)分别为104.6%和103.5%。结论:所建方法简便、快速,结果准确,为鸡骨草药材的质量评价提供了可靠的方法。
Objective ： To establish a method for the determination of abrine and hypaphorine in Abrus cantoniensis. Methods：An HPLC method was adopted. Chromatographic assay was performed on Kromasil Cls column（250 mm×4.6mm,5μm） at room temperature, with water - methanol - acetonitrile - glacial acetic acid - triethylamine（82： 13： 5： 0.2： 0. 3 ） as mobile phase. The flow rate was 1.0 mL · min^- 1 and the detection wavelength was 220 nm. Resuits：The abrine and hypaphorine had a good linearity in the range of 1.1 -55 mg · L^- 1 （r = 0. 9999 ） , the recoveries （ n = 6） were 104.6% and 103.5% , respectively. Conclusion ： The method is simple, accurate and reproducible. It can be used for quality control of Abrus cantoniensis.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2009年第10期1702-1704,共3页
Chinese Journal of Pharmaceutical Analysis
基金
广西科技攻关项目(0424008-2A)
作者简介
第一作者Tel：（0771）5868986；E—mail：hpingl208@yahoo．com．cn
