摘要
试验旨在研究枸杞多糖亚级分(LBP-4a)改善非胰岛素依赖型糖尿病(NIDDM)大鼠胰岛素抵抗(IR)的作用及其机制。试验采用水提醇沉法从宁夏枸杞中提取出枸杞多糖,并对其进行分离、纯化得主要活性成分LBP-4a。采用高糖高脂饮食结合小剂量腹腔注射链脲佐菌素(STZ)建立NIDDM大鼠模型,并将其分为模型对照组、LBP-4a低、高剂量组和罗格列酮治疗组,同时设立正常对照组。给药8周后检测相关指标。采用单细胞凝胶电泳技术对LBP-4a降糖、改善IR的作用机理进行了较为深入系统的探讨。结果表明,LBP-4a不仅对NIDDM大鼠具有降脂和增加抗氧化性的功能,还具有修复DNA损伤的作用。因此,LBP-4a作为一种可食的添加物能改善NIDDM大鼠的IR,进而预防NIDDM并发症及癌变的发生。
The experiment mean to explore the improving insulin resistance （IR） mechanism of Lycium barbarum polysaccharide 4a （LBP-4a） in non insulin dependent diabetes mellitus （NIDDM） rats. LBP was extracted from Ningxia-Lycium barbarum by water extraction and ethanol precipitation technique. And the ingredient of LBP-4a was obtained by separation and purification. The animal model of NIDDM was made by feeding high glucose and high-fat diet and subjecting to. i. p. little-dose streptozotocin （STZ）. The rats of NIDDM model were divided into four groups, including model group, LBP 4a low-dose and high -dose groups, rosiglitazone group and normal control group. In order to research the effect of LBP-4a on reducing blood glucose and ameliorating IR in NIDDM rats, a lot of techniques were used such as Single Cell Gel Electrophoresis et al. We studied systematically the effect of LBP-4a on IR in NIDDM rats and researched the mechanism. The results indicated that LBP-4a had lipid-lowering effects on NIDDM rats. Furthermore, LBP-4a had antioxidative function and repairing DNA damage on NIDDM rats. Therefore, LBP-4a as a dietary supplement may improve IR and prevent the development of complications or even tendency to carcinogenesis in NIDDM rats.
出处
《中国畜牧兽医》
CAS
北大核心
2009年第9期37-42,共6页
China Animal Husbandry & Veterinary Medicine
基金
大庆市科技局基金资助项目(2008SGG043)
关键词
枸杞多糖-4a
非胰岛素依赖型糖尿病
胰岛素抵抗
DNA损伤
lycium barbarum polysaccharide 4a
non insulin dependent diabetes mellitus
insulin resistance
DNA damage
作者简介
赵蕊（1975-），女，黑龙江人，讲师，博士，研究方向：中草药防治糖尿病的机制。
