摘要
目的:观察不同剂量枸杞多糖对D-半乳糖致衰老模型大鼠8-羟基鸟嘌呤糖苷酶表达的影响。方法:实验于2005-03/08在黑龙江省佳木斯大学生物化学教研室完成。①选用纯系Wistar大鼠40只。随机将大鼠分为4组:衰老组,枸杞多糖大剂量组,枸杞多糖中剂量组,枸杞多糖小剂量组,每组10只。衰老模型对照组:正常饮食,每日上午颈背部皮下按48mg/kg注射D-半乳糖,同时灌服温开水;枸杞多糖小剂量组、枸杞多糖中剂量组、枸杞多糖大剂量组:每组均正常饮食,除每日上午颈背部皮下按48mg/kg注射D-半乳糖外,同时分别按10,50,100mg/kg灌服枸杞多糖(市售枸杞多糖,用双蒸水分别制成10,50,100g/L溶液),连续给药45d。②用Trizol法提取肝组织核酸,反转录聚合酶链反应及凝胶成像技术检测8-羟基鸟嘌呤糖苷酶的mRNA表达水平。③计量资料差异比较采用方差分析和q检验。结果:大鼠40只均进入结果分析。大鼠肝脏组织的8-羟基鸟嘌呤糖苷酶mRNA表达水平:枸杞多糖小、中、大剂量组均明显低于衰老模型组(1.129±0.03,0.919±0.044,0.903±0.043,1.233±0.042,P<0.05),且随剂量的增加,呈下降趋势,枸杞多糖中剂量和大剂量组之间差异不明显。结论:枸杞多糖可以通过降低氧化损伤,减少DNA损伤,继而使修复酶8-羟基鸟嘌呤糖苷酶的表达下降而发挥抗衰老的作用。
AIM： To study the effect of lyeium barbarum polysaccharides at various dosages on expression of 8-oxoguanine in liver of aging model rats induced by D-galactose. METHODS： The experiment was completed in the Department of Biochemistry of College of Basic Medicine of Jiamusi University from March to August 2005. ① Forty pure-blood Wister rats were randomly divided into aging group, high, middle and low-dosage lycium barbarum polysacchrides groups with 10 in each group. Aging model control group： Rats were fed normally and were injected with 48 mg/kg D-galactose on the nap every morning, meanwhile, rats were perfused with warm boiled warter, Various dosage groups： Rats were fed normally and were injected with 48 mg/kg D galactose on the nap every morning, meanwhile, rats were perfused with 10, 50 and 100 mg/kg lycium barbarum polysaccharides for 45 days, Lycium barbarum polysaccharides in market were mixed with double-steamed water into 10, 50 and 100 g/L liquid respectively. ② Trizol reagent was applied to RNA extraction. Expressions of 8-oxoguanine mRNA were measured by reverse transcription polymerase chain reaction （RT-PCR） and gclatum imaging technique. ③ Measurement data were compared with analysis of wcriance and q test. RESULTS： All 40 rats were involved in the analysis of resuhs, Expressions of 8-oxoguanine mRNA in liver tissue： Expressions in various dosage groups were lower than those in aging model group （1.129±0.03, 0.919±0.044, 0.903±0.043, 1.233±0.042, P 〈 0.05）. The expression was decreased With the increase of dosages. There was no significant difference between middle and high dosage groups. CONCLUTION： Lycium barbarum polysaccharides can decrease DNA damagc through inhibiting oxidative injury, and then delay aging through decreasing the expression of 8-oxoguaniue repairase.
出处
《中国临床康复》
CAS
CSCD
北大核心
2006年第7期102-104,共3页
Chinese Journal of Clinical Rehabilitation
基金
黑龙江省研究生创新科研资金项目(YJSCX2005-114HLJ)~~
作者简介
李晶，女，1975年生，黑龙江省佳木斯市人，汉族，佳木斯大学在读硕士，讲师，主要从事中药抗衰老机制的研究。
通讯作者：欧琴，教授。佳木斯大学基础医学院生物化学教研室，黑龙江省佳木斯市154007
