摘要
目的:探讨核转录因子-κB(NF-κB)活性和肿瘤坏死因子(TNF-α)在脑缺血再灌注大鼠海马表达变化的规律和电针干预对其影响。方法:将SD大鼠随机分为正常组、假手术组、模型组、电针治疗组。采用改良线栓法制备局灶型脑缺血(MCAO)再灌模型,电针"大椎"、双侧"内关"穴。运用免疫组化检测法观察海马组织NF-κB-p65蛋白的表达及核转位;用放射免疫法测定各组大鼠海马组织中TNF-α含量的变化。结果:模型各组大鼠缺血侧海马CA1区NF-κB-p65蛋白表达和海马组织TNF-α含量显著增高(P<0.01)。与模型各组大鼠相比,电针治疗组缺血侧海马组织CA1区NF-κB-p65蛋白表达和TNF-α含量均显著降低。结论:TNF-α的表达上调后可活化NF-κB,参与脑损伤后继发性炎症反应过程,电针能抑制其活化可以减轻脑缺血再灌注时的炎症反应,发挥神经保护作用。
Objective：To investigate effects of acupuncture on the expression and content of nuclear factor-KB （NF- KB） and tumor necrosis faetor-α （TNF-α） in hippocampus of rat brain after cerebral isehemia/reperfusion. Method： SD rats were randomly divided into normal group, sham operation group,model group, and electroacupuncture group. The rat＇s middle cerebral artery was blocked to establish cerebral isehemia （MCAO）/reperfusion model with suturing MCA.＂Dazhui＂（BU14）,＂Neiguan＂（PC6） of both side were treated with electroaeupuncture. The expression and content of nuclear factor-KB in hippocampus were detected with immunohistochemistry and the content of TNF-α in hippocampus were detected by means of radio-immunoassay （RIA）. Result：The expression of NF-KB-p65 in ischemic hippocampi CA1 and content of TNF-α in hippocampus of rats in model group were significantly higher than those in electroacupuncture group （P〈0.01,〈0.05）. Conclusion： NF-KB and TNF-α may play an essential role in injury-induced inflammatory response that leads to secondary insults after brain injury.Eleetroacupuncture may inhibit the expression of NF-KB in hippocampus of rat brain after cerebral ischemia/reperfusion, reduce the inflammatory response and block it migrating into nucleus. Electroacupuncture can provide the protective effects on neuronal cells after cerebral isehemia/reperfusion in rats.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2009年第8期711-714,I0002,共5页
Chinese Journal of Rehabilitation Medicine
基金
湖北省自然科学基金课题(2003ABA154)
关键词
电针
脑缺血再灌注
海马
核转录因子
肿瘤坏死因子
eleetroacupuncture
cerebral ischemia reperfusion
hippocampus
nuclear factor-KB
tumor necrosis faetor-α
作者简介
作者简介：孔立红，女，副教授，博士
