摘要
样品经提取、过滤、离心后通过免疫亲和柱净化、衍生，荧光检测器检测。该方法灵敏度高，达到了国际上对食品中黄曲霉毒素B1的要求，外标法定量。黄曲霉毒素B1的检测限为0．1μg／kg，线性范围为1．0～45μg／kg，加标回收率为80％-110％，结果准确可靠。
A rapid analytical method for the determination of Aflatoxi B1 in Chinese herbal medicine extraction by high performance liquid chromatography （HPLC） with pre-eolumn derivatization had been developed. The Chinese herbal medicine was extracted by methanol and the extraction was cleaned up with a solid phase extraction column. The analytes was separated by using C18 column and acetonitfile-water as mobile phase with a flow rate of 0. 5 mL/min. The fluorescence detector was set at excitation wavelength 360 nm and emission wavelength were 440 rim. In the range of 1.0 -45μg/kg, fwerethere was good linear correlation between the concentrations and peak areas of analytes. The spiked recoveries were 80 % - 110 %. The limits of detection （ S/N = 3 ） was 0. 1μg/kg.
出处
《西南农业学报》
CSCD
北大核心
2009年第2期522-524,共3页
Southwest China Journal of Agricultural Sciences
关键词
高效液相色谱
柱前衍生
黄曲霉毒素B1
中草药提取物
High performance liquid chromatography
Precolumn derivatization
Aflatoxi Bt
Chinese herbal medicine
作者简介
付成平（1971-），男，四川平昌人，主要从事食品分析与真菌毒素的研究。
