摘要
应用敏感家蝇的乙酰胆碱酯酶（ＡｃｈＥ）检测了蔬菜中有机磷和氨基甲酸酯类农药的残留。若以抑制酶活性１６％的剂量为检出标准，对上述两类农药的灵敏度分别为０．０００２７～１．３６３２μｇ和０．００１５～０．５２０５μｇ。方法回收率为７０％～１０３％。对ＡｃｈＥ抑制法的实际应用效果进行田间验证，并结合生物测定法抽检了昆明的市售蔬菜。结果表明：在受农药污染的样本中，有５０％的污染是由有机磷和氨基甲酸酯类农药造成的。
The residues of organophosphates and carbamates in vegetables were tested by acetylcholinesterase (AchE) of sensitive house flies(Muxca domestica).If the standard of detection was a dosage of inhibiting 16% of AchE activety, sensitivities toorganphosphates and carbamates were 0.00027-1.3632μg and 0.0015-0.5025μg,respectively.The recoveries of the method ranged betweeh 70% and 103%.AchE's inhibitory method were confirmed in the field.In cooperation with bioassay,the samples of vegetables collected from Kunming markets were detected by the method.The results showed that 50% polluted vegetable samples came from abusing organophosphates and carbamates in the field.
出处
《西南农业学报》
CSCD
1996年第2期62-66,共5页
Southwest China Journal of Agricultural Sciences
基金
云南省应用基础研究基金
关键词
农药残留
蔬菜
生物化学检测
Acetylcholinesterase
Musca domestica
Pesticide residue
Vegetable
