摘要
目的:探讨IL-21对UC患者外周血和肠黏膜固有层组织内淋巴细胞的免疫病理调节.方法:收集28例UC患者和22例健康成人外周血标本和肠黏膜活检标本,分离外周血单个核淋巴细胞(PBMC)和肠黏膜固有层单个核淋巴细胞(LPMC),利用流式细胞仪检测IL-21R在CD4+、CD8+T细胞、B细胞和NK细胞表面表达.培养PBMC和LPMC,使用IL-21和抗CD3单抗体外刺激,48h后收集上清液,使用ELISA检测促炎症细胞因子(TNF-α、IFN-γ、IL-2)分泌,分析IL-21在疾病发展过程中的免疫病理作用.结果:UC患者外周血和肠黏膜固有层组织内CD4+、CD8+T细胞、CD20+B细胞和CD56+NK细胞表达IL-21R水平比健康者显著升高(PBMC:8.42±2.14vs3.46±0.54,10.35±2.17vs5.28±2.2,7.27±1.15vs2.35±0.41,12.55±3.12vs5.45±1.06;LPMC:22.44±3.46vs6.26±1.15,24.48±4.57vs6.87±1.02,16.24±3.10vs5.56±1.44,23.54±4.12vs8.45±1.68,均P<0.05).体外培养PBMC或LPMC,使用IL-21刺激,发现IL-21可显著诱导UC患者的PBMC和LPMC激活,并分泌高水平的TNF-α和IFN-γ(346±72vs120±27,3048±426vs1182±242;625±113vs154±35,3827±418vs1520±304,均P<0.05).结论:IL-21R参与肠黏膜炎症损伤,阻断IL-21生物学效应可能治疗UC的发生.
AIM： To explore the role of IL-21 receptor （IL- 21R） in peripheral blood mononuclear cells （PBMC） and lamina propria mononuclear cells （LPMC） for patients with ulcerative colitis （UC）. METHODS： Peripheral blood samples and colonic biopsies were obtained from 28 patients with UC and 22 healthy controls. Expression of IL-21R in peripheral blood or lamina propria CD4^＋, CD8^＋ T cells, B cells and NK cells was analyzed using flow cytometry. Cytokine secretion by PBMC or LPMC under stimulation with IL-21 and anti-CD3 was detected using ELISA.RESULTS： Expression of IL-21R was significantly higher in peripheral blood and lamina propria CD4^＋, CD8^＋ T ceils, B ceils and NK ceils of UC patients than in healthy controls （PBMC： 8.42 ± 2.14 vs 3.46 ± 0.54, 10.35 ± 2.17 vs 5.28 ± 2.2, 7.27 ± 1.15 vs 2.35 ± 0.41,12.55 ± 3.12 vs 5.45 ± 1.06; LPMC： 22.44 ± 3.46 vs 6.26 ± 1.15, 24.48 ± 4.57 i 6.87 ± 1.02, 16.24 ± 3.10 vs 5.56 ± 1.44, 23.54 ± 4.12 vs 8.45 ± 1.68, all P 〈 0.05）. PBMC or LPMC from UC patients, when stimulated with IL-21 and anti-CD3, produced significantly higher levels of TNF-α, IFN-γ and IL-2 compared with healthy controls （346 ± 72 vs 120 ± 27, 3048 ± 426 vs 1182 ± 242; 625 ± 113 vs 154 ± 35, 3827 ± 418 vs 1520 ± 304, all P 〈 0.05） CONCLUSION： IL-21R is significantly increased in PBMC and LPMC from UC patients, and IL-21 induces proinflamatory cytokine secretion. It suggests that IL-21 is associated with intestinal mucosal injury and that target therapy directed against IL-21 may be used to treat UC patients.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第1期102-105,共4页
World Chinese Journal of Digestology
基金
教育部新世纪优秀人才支持计划基金资助项目
NoNECT-05-0609
河南省卫生厅科技攻关基金资助项目
No200704009
作者简介
通讯作者：刘占举，450014，河南省郑州市经八路2号，郑州大学第二附属医院消化内科 zhanjuliu@yahoo.com. 电话：0371—63939084
