摘要
目的:以超滤法对伊立替康(CPT-11)脂质体包封率的测定方法进行研究。方法:采用薄膜蒸发-高压乳匀法制备CPT-11脂质体,以超滤法分离脂质体和游离药物,建立 HPLC 测定 CPT-11含量的方法。结果:超滤法能将脂质体与游离药物很好地分离,药物回收率为99.3%～100.9%,加样回收率为99.6%～102.5%。在选定色谱条件下,CPT-11分离良好,在0.106～1.012 mg·mL^(-1)范围内呈良好的线性关系(r=0.9996),日内 RSD 为1.6%,日间 RSD 为2.3%(n=5),回收率为97.7%～100.4%,。RSD 为1.1%～2.3%。结论:采用超滤-HPLC 法能较好测定 CPT-11脂质体的含量及包封率,同时具有简单、准确、快速的优点。
Objective ： To study the entrapment efficiency of irinotecan （ CPT - 11 ） liposome by ultrafiltration meth- od. Methods：Uhrafiltration method was employed to separate the free drug from the liposomes. The content of CPT - 11 was determined by a HPLC method. Results：The free drug was well separated from the liposome by ultrafiltra- tion method. The drug recovery by ultrafiltration was in a range of 99. 3% - 100.9% , the sample recovery was 99.6% - 102.5%, respectively. A calibrated linear curve of CPT - 11 concentration was within 0. 106 - 1. 012 mg · mL-1（ r =0. 9996） ,and RSD within day was 1.6% and RSD between days was 2.3% （ n =5） ,the recovery of CPT- 11 was in a range of 97.7% -100. 4% with RSD of 1.1% -2.3%. Conclusion：The ultrafiltration method used in this study is simple, accurate and applicable for determination entrapment efficiency of CPT - 11.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2008年第12期2081-2084,共4页
Chinese Journal of Pharmaceutical Analysis
关键词
超滤法
包封率
伊立替康
脂质体
ultrafihration
entrapment efficiency
irinotecan （ CPT - 11 ）
liposomes
作者简介
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