摘要
目的：建立测定文冠木中总黄酮的含量的方法。方法：采用紫外可见分光光度法测定,检测波长为525nm。结果：槲皮素在浓度6.657～33.286μg/mL范围内呈良好线性关系。回归方程Y=1.1749X＋0.008（r=0.9998）。线性关系良好,加样回收率为98.44%,RSD为1.18%。结论：该方法简便、灵敏、准确,可用于文冠木中总黄酮的含量测定。
Objective：To establish the content determination method for flavone in Xanthoceras sorbifolia Bunge. Methods： Using ultraviolet and visible spectrophotometric determination, detecting at wavelength of 525 nm. Results： Meletin had a liner relationship with the absorbance in the range of 6.657 - 33.2861μg/mL and the regression equation was as follows： Y=1. 1749X＋0.008 （r = 0. 9998 ）. The average recovery for meletin was 98.44%, RSD = 1.18%. Conclusion：The method is simple, sensitive,accurate and can be used for total flavones determination in Xanthoceras sorbifolia Bunge.
出处
《内蒙古医学院学报》
2008年第5期364-366,370,共4页
Acta Academiae Medicinae Neimongol
基金
内蒙古自治区高等学校科学研究项目(NJZY07090)
关键词
文冠木
总黄酮
紫外可见分光光度法
Xanthoceras sorbifolia Bunge
total flavone
UV - visible spectrophotometry
作者简介
董玉（1975-），女，内蒙古医学院药学院讲师，中药学博士。
