摘要
目的:观察中药复方脂肝乐(ZGL)对高脂饲料所致大鼠脂肪肝的治疗作用,并探讨其作用机制。方法:Wistar大鼠随机分为6组,除正常组外,其余各组均给予高脂饲料,连续12周,第13周开始,ZGL灌胃给药,持续12周。实验结束处死动物,检测血清甘油三酯(TG)、总胆固醇含量(T-CH);肝脏TG、T-CH、谷胱甘肽(GSH)、丙二醛(MDA)含量;肝脏病理检查,观察显微结构变化。结果:ZGL能显著降低血清T-CH、TG含量,降低肝脏T-CH、TG、MDA含量(P<0.01,P<0.05);升高肝脏GSH含量(P<0.01,P<0.05),病理学变化可得到明显改善。结论:脂肝乐有明显抗脂质过氧化、促进脂质从肝脏排除、减少脂质在肝内蓄积的作用,具有显著抗脂肪肝作用。
Objective： To explore the curative effect of Chinese herbal compounds, Zhiganle to hepatic adiposis in rats induced by high fat diet, and to study its mechanism. Method： Wistar rats were randomly divided into 6 groups： Normal control （ NC ）, pathological model （ PM ）, Dongbaogantai treated group （ DT）, and 3 Zhiganle treatment groups that received different dosages of Zhiganle （ ZT1 1.5 g·kg^-1·d^-1, T2 3.0 g·kg^-1·d^-1, and ZT3 4.5 g·kg^-1·d^-1 ）. All the experimental rats except those in group NC, were given high fat diet for 12 weeks and then fed with normal diet. Began in the 132 week, rats in group DT were administered with Dongbaogantai（0.3 g·kg^-1·d^-1 ）, and rats in groups ZT1, ZT2, and ZT3 received Zhiganle for other 12 weeks. Rats in NC were given normal saline instead. Finally, all the rats were executed, and their serum triglyceride （TG）, serum total cholesterol （T-CH）, hepatic TG, hepatic T-CH, glutathione （GSH）, malonaldehyde （MDA） and hepatic pathological changes were checked. Results： Levels of serum TG, serum T-CH, hepatic TG, hepatic T-CH, and MDA were clearly higher （P 〈0.01 or P 〈 0.05 ） and GSH level obviously lower （P 〈 0.01 or P 〈0.05） in group PM than those in group NC; the levels of serum TG, serum T-CH, hepatic TG, hepatic T-CH, and MDA in treatment groups were clearly lower （ P 〈 0.01 and P 〈 0.05） and GSH level in the liver remarkably higher （P 〈0.01 and P 〈0.05） than those in group PM. Hepatic pathological changes were lightened in treated groups than those in group PM. Conclusion： Zhiganle has good treating effects on hepatic adiposis in rats induced by high fat diet.
出处
《贵阳医学院学报》
CAS
2008年第5期478-480,484,共4页
Journal of Guiyang Medical College
