摘要
建立用乙醇抽提+沉淀吸附法提取山楂中熊果酸的工艺,并探讨山楂熊果酸对高脂血症小鼠的降血脂作用。先用乙醇反复浸提山楂匀浆得到粗品,再用D型大孔树脂二次提纯得精品,经鉴定纯度后将其制成颗粒为纳米级的乳浊液备用。采用高脂乳剂灌胃小白鼠建立高脂模型,观察两种给药途径下三个剂量水平的熊果酸对模型小鼠血清指标胆固醇(TC)、甘油三酯(TG)、HDL(高密度脂蛋白)、LDL(低密度脂蛋白)的影响。结果显示熊果酸经鉴定纯度达98%,三个剂量熊果酸能降低TC、TG指数。结论是熊果酸提取工艺可靠,熊果酸对高脂血症小鼠有一定的降脂作用。
This paper established the method for extracting ursolic acid（UA） from hawthorn fruits and explored hypolipidemic effect of UA on mice with hyperlipidemia. Firstly, the crude product UA was obtained by homogenate and alcohol leaching. Then the purity of UA was elevated by precipitation and macro-porous resin adsorption. The emulsion of nanosuspensions was achieved by nanoprecipitation method. Secondly, set up hyperlipidemia model with high-fat cream. Observe the influence of UA at different doses to the levels of serum TC ,TG, HDL and LDL. The purity of UA was 98% by evaluation. The effect of UA depends on the dose and administration route. So we can make the conclusion that the extracting method was reliable and available,and UA could decrease blood lipid to some extent.
出处
《天然产物研究与开发》
CAS
CSCD
2007年第6期1052-1054,共3页
Natural Product Research and Development
关键词
熊果酸
高脂乳剂
降血脂
TC
TG
ursolic acid
high-fat cream
hypolipidemic
TC
TG
作者简介
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