摘要
目的明确白癜风患者皮损处是否存在核因子E2p45相关因子2（Nrf2）核转位异常。方法应用试剂盒检测8例表皮移植白癜风患者皮损处与正常供皮处疱液中超氧化物歧化酶（SOD）、丙二醛（MDA）和过氧化氢酶（CAT）水平，采用细胞免疫荧光组化法鉴定8例寻常型白癜风患者皮损处与正常供皮处表皮细胞以及3例健康对照包皮原代培养的表皮细胞中Nrf2的表达，同时应用蛋白质印迹法检测上述样本中表皮细胞的胞质和胞核中Nrf2的表达水平。结果皮损处SOD与CAT水平显著低于正常供皮处（P〈0．05），MDA水平显著高于正常供皮处（P〈0．05）；细胞免疫荧光结果显示，健康对照、患者正常供皮处Nrf2在表皮细胞的胞质和胞核中均有表达，而患者皮损处Nrf2表达主要集中在胞质，胞核中表达量非常低。蛋白质印迹结果显示，核蛋白中Nrf2表达水平，白癜风患者皮损处（0．11±0．03）明显低于正常供皮处（0．27±0．06）和健康对照（0．32±0．02，均P〈0．01）；胞质蛋白Nrf2表达水平，皮损处（0．63±0．04）与正常供皮处（0．61±0．03）和健康对照（0．65±0．04）差异无统计学意义（均P〉0．05）。结论白癜风患者皮损处表皮细胞存在Nrf2核转位异常。
Objective To investigate whether abnormal translocation of nuclear factor-E2 related factor 2 （ Nrf2 ） exists in the lesion of vitiligo. Methods Skin specimens from 8 vitiligo patients and 3 healthy controls were collected, haft of them underwent laser co-focal microscopy to detect the Nrf2 location and half of them underwent cell culture. Blister fluid was collected form the 8 vitiligo patients and skin donor sites to detect the levels of serum superoxide dismutase （SOD）, catalase （CAT）, and melonyldialdehyde （MDA） by using detection kit. Expression of Nrtf2 in epidermal cell of the 8 vitiligo patients and primary epidermal cell of the 3 healthy controls was identified with cell immunofluorescence histochemistry method. The nuclear and cytoplasmic proteins of all above samples were isolated to be identified by Western blotting. Results The levels of SOD and CAT in the lesion tissue were significantly lower than those in the skin donor site. The levels of MDA in the lesion tissue were significantly higher than those in the skin donor sites （ both P 〈 0.05 ）. Immunofluorescence histochemistry, showed that Nrf2 was predominantly cytoplasmic in the epidermal cells in the lesion, while Nrf2 expression could be seen in both the cytoplasm and nucleus in the epidermal cells in the normal skin donor sites and skins of the healthy controls. Western blotting showed that the nuclear Nrf2 level in the vitiligo skin lesion was（0.11 ±0.03）, significantly lower than that in the normal skin donor site （0.27 ±0.06） and in the skins of the healthy controls （0.32 ±0.02） （both P 〈 0.01 ）. However, there was no significant difference in the Nrf2 level of in cytoplasm among the three types oftissues （0.63 ±0.04,0.61 ±0.03,and 0.65 ± 0.04,all P 〉0.05）. Conclusion Nrf2 does not translocate from cytoplasm into the nucleus in the lesion of vitiligo patients.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2008年第34期2403-2406,共4页
National Medical Journal of China
作者简介
通讯作者：关翠萍，Email：imgcp@hotmail．com
