摘要
目的:制作大鼠门静脉灌注标本,获取每一层面血管铸型数据,计算机辅助重建门静脉数字模型。方法:选用丙烯颜料为主要成分的填充剂,注入大鼠活体门静脉系统内(20例),得到大鼠门静脉灌注标本。包埋后冰冻切片,每一切面进行拍照,获取血管的层片图像数据。导入软件中进行图像分割与三维重建。结果:该方法能成功地制作出大鼠肝脏血管铸型标本,填充剂可灌注到直径50～100μm的终末小血管。图层间距50μm,每一标本可获取约200～300张层片,并通过三维重建可视化显示。结论:此方法是计算机重建大鼠肝血管树数字模型的一种可行方法。
Objective：The casting sample of rat liver was fabricated, and the data of every section was harvested. The 3D-reconstruction of digital model of portal veins was reconstructed with computer and software. Methods ：The propylene pigment preparation was injected into the rat portal vein （20 cases）. The sample was embedded and cut into frozen sections. The data of every section of rat liver was collected by digital camera. Image segmentation and 3D-reconstruction were applied in photos. Results：Eighteen cases of the casting sample of rat liver were made successfully by this method. The propylene pigment preparation can enter small blood vessel whose diameter was 50-100 μm. Every case had about 200-300 photographs of the sections by an interval of 50 μm. They can be showed by computer vision based three-dimensional reconstruction. Conclusion：The method described above is feasible to get experimental data for 3D-reconstruction of digital model of rat liver and portal veins.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第8期977-979,1047,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
南京市科技发展项目(200702073)
关键词
大鼠
三位重建
门静脉
血管铸型
形态学
rat
three-dimensional reconstruction
portal veins
vascular casting
morphology
作者简介
通信作者。E-mail：sunxitai@sohu．com
