摘要
目的 明确Wistar大鼠肝脏声像图表现，探讨大鼠肝脏在超声医学实验研究中的应用。方法 Wistar大鼠40只，利用高频探头观察肝脏二维图像；将大鼠随机分为2组，每组20只，超声监视下经皮将美蓝溶液（0．05～0．1ml／只）注入肝左外叶（组1）或右侧各分叶（组2）；尾静脉团注超声造影剂观察肝脏造影过程；处死动物，检查肝叶间有无美蓝溶液渗出；摘除肝脏，测量各分叶。结果 在高频探头下隐约可见大鼠肝脏各叶的纤细分界；组1无美蓝渗出，组2有5例发生渗出；所有病例可清晰显示肝脏微泡造影的动脉相、门静脉相和延迟相。结论高频探头可以区分Wistar大鼠肝脏的部分分叶，肝左外叶易于在超声下辨认，对之进行穿刺操作时，不易发生刺透现象；大鼠肝脏可用于微泡造影剂成像等超声实验研究，但其动脉相和门静脉相持续时间短，针对这两个时相的研究可能会受到一定的影响。
Objective To confirm the ultrasound findings of the liver in normal Wistar rats and explore its＇ application in ultrasonic experiments. Methods Forty adult Wistar rats weighted about 200 g were studied. Two-dimensional ultrasound with high frequency scan head and CDFI were used to detect the liver of rats after anesthesia. The rats were randomly divided into 2 groups. Methylene blue about 0.05-0.1 ml were injected into left exterior hepatic lobe of rats in group 1 and right hepatic lobes in group 2 individually; Pulse inversion harmonic imaging was used to evaluate the process of enhancement of liver after bolus injection of ultrasonic contrast agent（0.05 ml/200 g） through caudal vein; The rats were then killed and examined if there were leaking out of methylene blue between hepatic lobes; The size of each lobes was also studied with slide gauge. Results The liver of Wistar rats contains 6 lobes： left exterior lobe, left interior lobe, median lobe, right lobe, caudate lobe and papillary lobe; There was not leaking out of methylene blue in cases of group 1, while 5/20 cases were found leaking out in group 2; Hepatic artery stage, portal vein stage and balance stage last for 2-4 s, 3-8 s and 3-5 rain individually could be seen clearly during contrast enhancement of liver in all the cases. Conclusion High-frequency uhrasonography can be used to discern parts of hepatic lobes in Wistar rats. Left exterior lobe was easily identified on ultrasound imaging. Penetration seldom happened during puncture operations on left exterior lobe; The liver of rats could be used in experiment of contrast enhancement imaging. While, the relatively short duration of artery stage and portal vein stage may limit the use of Wistar rats in the experiment of hepatic tumor contrast enhancement to some extent.
出处
《中国医学影像技术》
CSCD
北大核心
2006年第1期39-41,共3页
Chinese Journal of Medical Imaging Technology
基金
湖北省自然科学基金项目资助(2004ABA247)。
关键词
大鼠
肝脏
超声检查
微泡
造影剂
Rats
Liver
Ultrasonography
Microbubbles
Contrast media
作者简介
张超（1976-），男，辽宁锦州人，博士，主治医师。研究方向：腹部疾病超声诊断。E-mail：zhchjacky@hotmail．com
[通讯作者]黄道中，华中科技大学同济医学院附属同济医院超声影像科，430030。E-mail：HDZ@hotmail．com
