摘要
本试验用富含葡萄球菌Ａ蛋白的国际标准菌株ｃｏｗａｎⅠ分别标记３株引起鱼类致病的荧光假单胞菌、鳗弧菌和鲁克氏耶尔森菌的特异高免血清制成ＳＰＡ诊断液，建立了检测这３株病原菌的协同凝集试验。将待检病料培养１６—１８ｈ，用该法检测３ｍｉｎ可判定结果，比常规玻片法敏感２５—５０倍；经交叉试验和重复性试验，表明特异性强，敏感性高，可重复性好，且具有简便快速经济等特点。适用于基层鱼场，可用于淡水鱼类病原菌的快速鉴定、疫情预报和流行病学调查。
オabbit antiserum of Psudomonas fluorescens,Yersinia ruckeri and Vibril anguillarum were labbelled with staphylococcal Protein A(SPA) of cowan I,and was made diagnostic reagent,then co-agglutination test (COA)was established.The detected samples were inoculated into tryptic soy broth for 16-18h,and were identified by SPA-COA in 3 min,it was 25-50 times more sensitive than routine slide agglutination test.Cross reaction and repeat test showed that the SPA-COA was specific,sensitive and can be repeated.It was simple,rapid,economical and can be used in fish farm.The SPA-COA is able to detect and identify the pathogenic bacteria from fish rapidly.It can also be used to investigate and forcast epidemic disease caused by the bacteria.
出处
《水产科学》
CAS
北大核心
1997年第6期12-15,共4页
Fisheries Science
