摘要
本文采用自制的ＲＳＶ免疫血清以及国产试剂，通过特异性、敏感度和重复性试验，建立了检测ＲＳＶ抗原的生物素－亲和素－酶联兔疫吸附试验（ＢＡ－ＥＬＩＳＡ）。该法比常规的ＥＬＩＳＡ法敏感４倍。用此法检测５８份肺炎和毛细支气管炎患儿鼻咽分泌物（ＮＰＳ）中的ＲＳＶ抗原，结果与组织培养（ＴＣ）分离ＲＳＶ结果比较：其特异性和敏感性分别为９１．２％和９５．８％，两法诊断符合率为９３．１％。结果表明本法不但敏感、特异，而且简便、快速、经济，在收到标本２４小时即可得到结果，是一种适应于基层推广的ＲＳＶ感染诊断方法。
A biotin-avidin-enzyme-linked immunosorbent assay for detection of respiratory syncytial virus (RSV) antigens was developed, using antisera made in our institute. Guinea pig and rabbit anti--RSV immunoglobulins were uSed as capture and detector antibodies respectively.The specificity of this method was determined by crossing and blocking tests, revealing the sensitivity being 4 times higher than that ofconventional ELISA.Applying the assay to decteet RSV in 58 NPS specipsens, thespecficity and sensitivity were 91. ZN and 95.8% respectively.The result showed thatthe assay could be easily performed in 24h and was highly sensitiye and specific.
出处
《上海免疫学杂志》
CSCD
北大核心
1994年第2期100-102,共3页
Shanghai Journal of Immunology
